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Nodal Promotes the Self-Renewal of Human Colon Cancer Stem Cells via an Autocrine Manner through Smad23 Signaling Pathway
Joint Authors
Guo, Ying
Zhang, Zhenzhen
He, Zuping
Yang, Shi
Ma, Meng
Liu, Linhong
Li, Zheng
Liu, Yang
Yang, Hao
Hai, Yanan
Gong, Yuehua
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Colorectal cancer is one of the most common and fatal tumors.
However, molecular mechanisms underlying carcinogenesis of colorectal cancer remain largely undefined.
Here, we explored the expression and function of Nodal in colon cancer stem cells (CCSCs).
Nodal and its receptors were present in numerous human colorectal cancer cell lines.
NODAL and ALK-4 were coexpressed in human colon cancerous tissues, and NODAL, CD24, and CD44, markers for CCSCs, were expressed at higher levels in human colon cancerous tissues than adjacent noncancerous colon tissues.
Human CCSCs were isolated by magnetic activated cell sorting using anti-CD24 and anti-CD44.
Nodal transcript and protein were hardly detectable in CD44- or CD24-negative human colorectal cancer cell lines, whereas Nodal and its receptors were present in CCSCs.
Notably, Nodal facilitated spheroid formation of human CCSCs, and phosphorylation of Smad2 and Smad3 was activated by Nodal in cells of spheres derived from human CCSCs.
Collectively, these results suggest that Nodal promotes the self-renewal of human CCSCs and mediate carcinogenesis of human colorectal cancer via an autocrine manner through Smad2/3 pathway.
This study provides a novel insight into molecular mechanisms controlling fate of human CCSCs and offers new targets for gene therapy of human colorectal cancer.
American Psychological Association (APA)
Gong, Yuehua& Guo, Ying& Hai, Yanan& Yang, Hao& Liu, Yang& Yang, Shi…[et al.]. 2014. Nodal Promotes the Self-Renewal of Human Colon Cancer Stem Cells via an Autocrine Manner through Smad23 Signaling Pathway. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-466119
Modern Language Association (MLA)
Gong, Yuehua…[et al.]. Nodal Promotes the Self-Renewal of Human Colon Cancer Stem Cells via an Autocrine Manner through Smad23 Signaling Pathway. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-466119
American Medical Association (AMA)
Gong, Yuehua& Guo, Ying& Hai, Yanan& Yang, Hao& Liu, Yang& Yang, Shi…[et al.]. Nodal Promotes the Self-Renewal of Human Colon Cancer Stem Cells via an Autocrine Manner through Smad23 Signaling Pathway. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-466119
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466119