Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
Joint Authors
Knežević-Jugović, Zorica
Lučić Škorić, Marija
Milašinović, Nikola
Filipović, Jovanka
Milosavljević, Nedeljko
Kalagasidis Krušić, Melina
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release.
By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine.
The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology.
The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules.
Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences.
The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution.
The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.
American Psychological Association (APA)
Milašinović, Nikola& Knežević-Jugović, Zorica& Milosavljević, Nedeljko& Lučić Škorić, Marija& Filipović, Jovanka& Kalagasidis Krušić, Melina. 2014. Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-466198
Modern Language Association (MLA)
Milašinović, Nikola…[et al.]. Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-466198
American Medical Association (AMA)
Milašinović, Nikola& Knežević-Jugović, Zorica& Milosavljević, Nedeljko& Lučić Škorić, Marija& Filipović, Jovanka& Kalagasidis Krušić, Melina. Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-466198
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466198