Extracellular Matrix Proteins Expression Profiling in Chemoresistant Variants of the A2780 Ovarian Cancer Cell Line
Joint Authors
Januchowski, Radosław
Ruciński, Marcin
Nowicki, Michał
Zawierucha, Piotr
Zabel, Maciej
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Ovarian cancer is the leading cause of death among gynaecological malignancies.
Extracellular matrix (ECM) can affect drug resistance by preventing the penetration of the drug into cancer cells and increased resistance to apoptosis.
This study demonstrates alterations in the expression levels of ECM components and related genes in cisplatin-, doxorubicin-, topotecan-, and paclitaxel-resistant variants of the A2780 ovarian cancer cell line.
Affymetrix Gene Chip Human Genome Array Strips were used for hybridisations.
The genes that had altered expression levels in drug-resistant sublines were selected and filtered by scatter plots.
The genes that were up- or downregulated more than fivefold were selected and listed.
Among the investigated genes, 28 genes were upregulated, 10 genes were downregulated, and two genes were down- or upregulated depending on the cell line.
Between upregulated genes 12 were upregulated very significantly—over 20-fold.
These genes included COL1A2, COL12A1, COL21A1, LOX, TGFBI, LAMB1, EFEMP1, GPC3, SDC2, MGP, MMP3, and TIMP3.
Four genes were very significantly downregulated: COL11A1, LAMA2, GPC6, and LUM.
The expression profiles of investigated genes provide a preliminary insight into the relationship between drug resistance and the expression of ECM components.
Identifying correlations between investigated genes and drug resistance will require further analysis.
American Psychological Association (APA)
Januchowski, Radosław& Zawierucha, Piotr& Ruciński, Marcin& Nowicki, Michał& Zabel, Maciej. 2014. Extracellular Matrix Proteins Expression Profiling in Chemoresistant Variants of the A2780 Ovarian Cancer Cell Line. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-466295
Modern Language Association (MLA)
Januchowski, Radosław…[et al.]. Extracellular Matrix Proteins Expression Profiling in Chemoresistant Variants of the A2780 Ovarian Cancer Cell Line. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-466295
American Medical Association (AMA)
Januchowski, Radosław& Zawierucha, Piotr& Ruciński, Marcin& Nowicki, Michał& Zabel, Maciej. Extracellular Matrix Proteins Expression Profiling in Chemoresistant Variants of the A2780 Ovarian Cancer Cell Line. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-466295
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466295