![](/images/graphics-bg.png)
Point Mutations in the folP Gene Partly Explain Sulfonamide Resistance of Streptococcus mutans
Joint Authors
Rwenyonyi, Charles Mugisha
Kironde, Fred
William, Buwembo
Swedberg, Göte
Aery, S.
Source
International Journal of Microbiology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-25
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Cotrimoxazole inhibits dhfr and dhps and reportedly selects for drug resistance in pathogens.
Here, Streptococcus mutans isolates were obtained from saliva of HIV/AIDS patients taking cotrimoxazole prophylaxis in Uganda.
The isolates were tested for resistance to cotrimoxazole and their folP DNA (which encodes sulfonamide-targeted enzyme dhps) cloned in pUC19.
A set of recombinant plasmids carrying different point mutations in cloned folP were separately transformed into folP-deficient Escherichia coli.
Using sulfonamide-containing media, we assessed the growth of folP-deficient bacteria harbouring plasmids with differing folP point mutations.
Interestingly, cloned folP with three mutations (A37V, N172D, R193Q) derived from Streptococcus mutans 8 conferred substantial resistance against sulfonamide to folP-deficient bacteria.
Indeed, change of any of the three residues (A37V, N172D, and R193Q) in plasmid-encoded folP diminished the bacterial resistance to sulfonamide while removal of all three mutations abolished the resistance.
In contrast, plasmids carrying four other mutations (A46V, E80K, Q122H, and S146G) in folP did not similarly confer any sulfonamide resistance to folP-knockout bacteria.
Nevertheless, sulfonamide resistance (MIC = 50 μM) of folP-knockout bacteria transformed with plasmid-encoded folP was much less than the resistance (MIC = 4 mM) expressed by chromosomally-encoded folP.
Therefore, folP point mutations only partially explain bacterial resistance to sulfonamide.
American Psychological Association (APA)
William, Buwembo& Aery, S.& Rwenyonyi, Charles Mugisha& Swedberg, Göte& Kironde, Fred. 2013. Point Mutations in the folP Gene Partly Explain Sulfonamide Resistance of Streptococcus mutans. International Journal of Microbiology،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-466309
Modern Language Association (MLA)
William, Buwembo…[et al.]. Point Mutations in the folP Gene Partly Explain Sulfonamide Resistance of Streptococcus mutans. International Journal of Microbiology No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-466309
American Medical Association (AMA)
William, Buwembo& Aery, S.& Rwenyonyi, Charles Mugisha& Swedberg, Göte& Kironde, Fred. Point Mutations in the folP Gene Partly Explain Sulfonamide Resistance of Streptococcus mutans. International Journal of Microbiology. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-466309
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466309