![](/images/graphics-bg.png)
Antibody-Hapten Recognition at the Surface of Functionalized Liposomes Studied by SPR : Steric Hindrance of Pegylated Phospholipids in Stealth Liposomes Prepared for Targeted Radionuclide Delivery
Joint Authors
Maillasson, Mike
Remaud-Le Saëc, Patricia
Faivre-Chauvet, Alain
Barbet, Jacques
Botosoa, Eliot. P.
Gestin, Jean-François
Jacques, Yannick
Mougin-Degraef, Marie
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Targeted PEGylated liposomes could increase the amount of drugs or radionuclides delivered to tumor cells.
They show favorable stability and pharmacokinetics, but steric hindrance of the PEG chains can block the binding of the targeting moiety.
Here, specific interactions between an antihapten antibody (clone 734, specific for the DTPA-indium complex) and DTPA-indium-tagged liposomes were characterized by surface plasmon resonance (SPR).
Non-PEGylated liposomes fused on CM5 chips whereas PEGylated liposomes did not.
By contrast, both PEGylated and non-PEGylated liposomes attached to L1 chips without fusion.
SPR binding kinetics showed that, in the absence of PEG, the antibody binds the hapten at the surface of lipid bilayers with the affinity of the soluble hapten.
The incorporation of PEGylated lipids hinders antibody binding to extents depending on PEGylated lipid fraction and PEG molecular weight.
SPR on immobilized liposomes thus appears as a useful technique to optimize formulations of liposomes for targeted therapy.
American Psychological Association (APA)
Botosoa, Eliot. P.& Maillasson, Mike& Mougin-Degraef, Marie& Remaud-Le Saëc, Patricia& Gestin, Jean-François& Jacques, Yannick…[et al.]. 2011. Antibody-Hapten Recognition at the Surface of Functionalized Liposomes Studied by SPR : Steric Hindrance of Pegylated Phospholipids in Stealth Liposomes Prepared for Targeted Radionuclide Delivery. Journal of Drug Delivery،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-466469
Modern Language Association (MLA)
Botosoa, Eliot. P.…[et al.]. Antibody-Hapten Recognition at the Surface of Functionalized Liposomes Studied by SPR : Steric Hindrance of Pegylated Phospholipids in Stealth Liposomes Prepared for Targeted Radionuclide Delivery. Journal of Drug Delivery No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-466469
American Medical Association (AMA)
Botosoa, Eliot. P.& Maillasson, Mike& Mougin-Degraef, Marie& Remaud-Le Saëc, Patricia& Gestin, Jean-François& Jacques, Yannick…[et al.]. Antibody-Hapten Recognition at the Surface of Functionalized Liposomes Studied by SPR : Steric Hindrance of Pegylated Phospholipids in Stealth Liposomes Prepared for Targeted Radionuclide Delivery. Journal of Drug Delivery. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-466469
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466469