Obesity and the Endometrium : Adipocyte-Secreted Proinflammatory TNFα Cytokine Enhances the Proliferation of Human Endometrial Glandular Cells
Joint Authors
Nguyen, HoVan
Salama, Salama
Nair, Sangeeta
al-Hendy, Ayman
Source
Obstetrics and Gynecology International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Obesity, a state of chronic inflammation, is associated with poor fertility and low implantation rates and is a well-documented risk factor for endometrial cancer.
Adipokines, such as tumor necrosis factor alpha, play an important role in initiation of endometrial cancer.
The aim of this study is to evaluate in vitro effects of human adipocyte cells (SW872) on growth of endometrial glandular epithelial cells (EGE).
Methods.
We measured cell proliferation and expression of cell-growth proteins—proliferating cell nuclear antigen, cyclin D1, cyclin-dependent kinase-1, and apoptotic markers (BCL-2 and BAK) in human EGE cells cocultured with SW872 cells.
EGE cells were also evaluated in SW872-conditioned media neutralized with anti-TNFα antibody.
Results.
A significant increase in EGE cell proliferation was observed in both SW872-conditioned media and in coculture (P<0.05).
We observed an upregulation of proliferation markers PCNA, cyclin D1, CDK-1, and BCL-2 and decrease in BAK (P<0.05).
Neutralization of SW872-conditioned media using anti-TNFα antibodies reversed EGE cell proliferation as indicated by BCL-2 expression.
Conclusions.
Adipocytes have potent proliferative paracrine effect on EGE cells which may be, in part, mediated via TNFα.
Further understanding of the role of obesity in endometrial carcinogenesis should lead to better preventative and therapeutic strategies.
American Psychological Association (APA)
Nair, Sangeeta& Nguyen, HoVan& Salama, Salama& al-Hendy, Ayman. 2013. Obesity and the Endometrium : Adipocyte-Secreted Proinflammatory TNFα Cytokine Enhances the Proliferation of Human Endometrial Glandular Cells. Obstetrics and Gynecology International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-466471
Modern Language Association (MLA)
Nair, Sangeeta…[et al.]. Obesity and the Endometrium : Adipocyte-Secreted Proinflammatory TNFα Cytokine Enhances the Proliferation of Human Endometrial Glandular Cells. Obstetrics and Gynecology International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-466471
American Medical Association (AMA)
Nair, Sangeeta& Nguyen, HoVan& Salama, Salama& al-Hendy, Ayman. Obesity and the Endometrium : Adipocyte-Secreted Proinflammatory TNFα Cytokine Enhances the Proliferation of Human Endometrial Glandular Cells. Obstetrics and Gynecology International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-466471
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466471