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Efficient Nonviral Gene Therapy Using Folate-Targeted Chitosan-DNA Nanoparticles In Vitro
Joint Authors
Wang, Huijie
Shi, Qin
Dai, Kerong
Zhang, Xiaoling
Qiu, Xingping
Winnik, Françoise M.
Fernandes, Julio C.
Jreyssaty, Christian
Benderdour, Mohamed
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Pharmacy, Health & Medical Sciences
Pharmacology
Abstract EN
Nonviral cationic polymers like chitosan can be combined with DNA to protect it from degradation.
The chitosan is a biocompatible, biodegradable, nontoxic, and cheap polycationic polymer with low immunogenicity.
The objective of this study was to synthesize and then assess different chitosan-DNA nanoparticles and to select the best ones for selective in vitro transfection in human epidermoid carcinoma (KB) cell lines.
It revealed that different combinations of molecular weight, the presence or absence of folic acid ligand, and different plasmid DNA sizes can lead to nanoparticles with various diameters and diverse transfection efficiencies.
The intracellular trafficking, nuclear uptake, and localization are also studied by confocal microscopy, which confirmed that DNA was delivered to cell nuclei to be expressed.
American Psychological Association (APA)
Jreyssaty, Christian& Shi, Qin& Wang, Huijie& Qiu, Xingping& Winnik, Françoise M.& Zhang, Xiaoling…[et al.]. 2012. Efficient Nonviral Gene Therapy Using Folate-Targeted Chitosan-DNA Nanoparticles In Vitro. ISRN Pharmaceutics،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-466537
Modern Language Association (MLA)
Jreyssaty, Christian…[et al.]. Efficient Nonviral Gene Therapy Using Folate-Targeted Chitosan-DNA Nanoparticles In Vitro. ISRN Pharmaceutics No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-466537
American Medical Association (AMA)
Jreyssaty, Christian& Shi, Qin& Wang, Huijie& Qiu, Xingping& Winnik, Françoise M.& Zhang, Xiaoling…[et al.]. Efficient Nonviral Gene Therapy Using Folate-Targeted Chitosan-DNA Nanoparticles In Vitro. ISRN Pharmaceutics. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-466537
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466537