Bioseparation of Four Proteins from Euphorbia characias Latex : Amine Oxidase, Peroxidase, Nucleotide PyrophosphatasePhosphodiesterase, and Purple Acid Phosphatase
Joint Authors
Medda, Rosaria
Floris, Giovanni
Pintus, Francesca
Spanò, Delia
Source
Biochemistry Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This paper deals with the purification of four proteins from Euphorbia characias latex, a copper amine oxidase, a nucleotide pyrophosphatase/phosphodiesterase, a peroxidase, and a purple acid phosphatase.
These proteins, very different in molecular weight, in primary structure, and in the catalyzed reaction, are purified using identical preliminary steps of purification and by chromatographic methods.
In particular, the DEAE-cellulose chromatography is used as a useful purification step for all the four enzymes.
The purification methods here reported allow to obtain a high purification of all the four proteins with a good yield.
This paper will give some thorough suggestions for researchers busy in separation of macromolecules from different sources.
American Psychological Association (APA)
Medda, Rosaria& Pintus, Francesca& Spanò, Delia& Floris, Giovanni. 2011. Bioseparation of Four Proteins from Euphorbia characias Latex : Amine Oxidase, Peroxidase, Nucleotide PyrophosphatasePhosphodiesterase, and Purple Acid Phosphatase. Biochemistry Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-466566
Modern Language Association (MLA)
Medda, Rosaria…[et al.]. Bioseparation of Four Proteins from Euphorbia characias Latex : Amine Oxidase, Peroxidase, Nucleotide PyrophosphatasePhosphodiesterase, and Purple Acid Phosphatase. Biochemistry Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-466566
American Medical Association (AMA)
Medda, Rosaria& Pintus, Francesca& Spanò, Delia& Floris, Giovanni. Bioseparation of Four Proteins from Euphorbia characias Latex : Amine Oxidase, Peroxidase, Nucleotide PyrophosphatasePhosphodiesterase, and Purple Acid Phosphatase. Biochemistry Research International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-466566
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466566