Singular Value Decomposition and Ligand Binding Analysis
Joint Authors
Colombo, Márcio Francisco
Galo, André Luiz
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Singular values decomposition (SVD) is one of the most important computations in linear algebra because of its vast application for data analysis.
It is particularly useful for resolving problems involving least-squares minimization, the determination of matrix rank, and the solution of certain problems involving Euclidean norms.
Such problems arise in the spectral analysis of ligand binding to macromolecule.
Here, we present a spectral data analysis method using SVD (SVD analysis) and nonlinear fitting to determine the binding characteristics of intercalating drugs to DNA.
This methodology reduces noise and identifies distinct spectral species similar to traditional principal component analysis as well as fitting nonlinear binding parameters.
We applied SVD analysis to investigate the interaction of actinomycin D and daunomycin with native DNA.
This methodology does not require prior knowledge of ligand molar extinction coefficients (free and bound), which potentially limits binding analysis.
Data are acquired simply by reconstructing the experimental data and by adjusting the product of deconvoluted matrices and the matrix of model coefficients determined by the Scatchard and McGee and von Hippel equation.
American Psychological Association (APA)
Galo, André Luiz& Colombo, Márcio Francisco. 2013. Singular Value Decomposition and Ligand Binding Analysis. Journal of Spectroscopy،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-466865
Modern Language Association (MLA)
Galo, André Luiz& Colombo, Márcio Francisco. Singular Value Decomposition and Ligand Binding Analysis. Journal of Spectroscopy No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-466865
American Medical Association (AMA)
Galo, André Luiz& Colombo, Márcio Francisco. Singular Value Decomposition and Ligand Binding Analysis. Journal of Spectroscopy. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-466865
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466865