Investigation of Antimicrobial Activity and Statistical Optimization of Bacillus subtilis SPB1 Biosurfactant Production in Solid-State Fermentation
Joint Authors
Saadaoui, Imen
Ayadi, Imen
Maktouf, Sameh
Chaabouni-Ellouze, Semia
Mnif, Ines
Chaabouni-Ellouze, Semia
Kammoun, Radhouan
Abdelkefi-Mesrati, Lobna
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
During the last years, several applications of biosurfactants with medical purposes have been reported.
Biosurfactants are considered relevant molecules for applications in combating many diseases.
However, their use is currently extremely limited due to their high cost in relation to that of chemical surfactants.
Use of inexpensive substrates can drastically decrease its production cost.
Here, twelve solid substrates were screened for the production of Bacillus subtilis SPB1 biosurfactant and the maximum yield was found with millet.
A Plackett-Burman design was then used to evaluate the effects of five variables (temperature, moisture, initial pH, inoculum age, and inoculum size).
Statistical analyses showed that temperature, inoculum age, and moisture content had significantly positive effect on SPB1 biosurfactant production.
Their values were further optimized using a central composite design and a response surface methodology.
The optimal conditions of temperature, inoculum age, and moisture content obtained under the conditions of study were 37°C, 14 h, and 88%, respectively.
The evaluation of the antimicrobial activity of this compound was carried out against 11 bacteria and 8 fungi.
The results demonstrated that this biosurfactant exhibited an important antimicrobial activity against microorganisms with multidrug-resistant profiles.
Its activity was very effective against Staphylococcus aureus, Staphylococcus xylosus, Enterococcus faecalis, Klebsiella pneumonia, and so forth.
American Psychological Association (APA)
Chaabouni-Ellouze, Semia& Abdelkefi-Mesrati, Lobna& Mnif, Ines& Kammoun, Radhouan& Ayadi, Imen& Saadaoui, Imen…[et al.]. 2012. Investigation of Antimicrobial Activity and Statistical Optimization of Bacillus subtilis SPB1 Biosurfactant Production in Solid-State Fermentation. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-466945
Modern Language Association (MLA)
Chaabouni-Ellouze, Semia…[et al.]. Investigation of Antimicrobial Activity and Statistical Optimization of Bacillus subtilis SPB1 Biosurfactant Production in Solid-State Fermentation. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-466945
American Medical Association (AMA)
Chaabouni-Ellouze, Semia& Abdelkefi-Mesrati, Lobna& Mnif, Ines& Kammoun, Radhouan& Ayadi, Imen& Saadaoui, Imen…[et al.]. Investigation of Antimicrobial Activity and Statistical Optimization of Bacillus subtilis SPB1 Biosurfactant Production in Solid-State Fermentation. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-466945
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466945