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Initial Report on Differential Expression of Sprouty Proteins 1 and 2 in Human Epithelial Ovarian Cancer Cell Lines
Joint Authors
Moghaddam, Samar Masoumi
Amini, Afshin
Pourgholami, Mohammad Hossein
Morris, David Lawson
Wei, Ai-Qun
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Sprouty (Spry) proteins, modulators of receptor tyrosine kinase signaling pathways, have been shown to be deregulated in a variety of pathological conditions including cancer.
In the present study we investigated the expression of Spry1 and Spry2 isoforms in a panel of human ovarian cancer cell lines in vitro.
Our western blot analysis showed nonuniform patterns of Spry expression in the cancer cells, none of which conformed to the pattern observed in the normal ovarian epithelial cells employed as the control.
Among the seven cancer cell lines studied, Spry1 was expressed lower in four cell lines and higher in one as compared with the control.
As for Spry2, four cell lines showed lower and two exhibited higher expression.
Results from RT-PCR assay raised the possibility that Spry protein levels may not necessarily correspond with its expression at mRNA level.
Our immunostaining study revealed that Spry2 was predominantly distributed within the whole cytoplasm in vesicular structures whereas Spry1 was found in both the cytoplasm and nucleus.
This might provide clues to further investigation of Spry mode of action and/or function.
Collectively, our study unveiled the differential expression of Spry1 and Spry2 proteins in various ovarian cancer cell lines.
American Psychological Association (APA)
Moghaddam, Samar Masoumi& Amini, Afshin& Wei, Ai-Qun& Pourgholami, Mohammad Hossein& Morris, David Lawson. 2012. Initial Report on Differential Expression of Sprouty Proteins 1 and 2 in Human Epithelial Ovarian Cancer Cell Lines. Journal of Oncology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-466962
Modern Language Association (MLA)
Moghaddam, Samar Masoumi…[et al.]. Initial Report on Differential Expression of Sprouty Proteins 1 and 2 in Human Epithelial Ovarian Cancer Cell Lines. Journal of Oncology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-466962
American Medical Association (AMA)
Moghaddam, Samar Masoumi& Amini, Afshin& Wei, Ai-Qun& Pourgholami, Mohammad Hossein& Morris, David Lawson. Initial Report on Differential Expression of Sprouty Proteins 1 and 2 in Human Epithelial Ovarian Cancer Cell Lines. Journal of Oncology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-466962
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466962