Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling
Joint Authors
Xing, Weiwei
Sun, Jinhao
Fan, Shangchun
Wang, Dongxue
Li, Cheng
Source
Journal of Applied Mathematics
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-26
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
We report a principle prototype of space animal cell perfusion culture and observation.
Unlike previous work, our cell culture system cannot only realize microfluidic and temperature controlling, automatic observation, and recording but also meet an increasing cell culture at large scale operation and overcome shear force for animal cells.
A key component in the system is ingenious structural fused silica cell culture cavity with the wedge-shaped connection.
Finite volume method (FVM) is applied to calculate its multipoint flow field, pressure field, axial velocity, tangential velocity, and radial velocity.
In order to provide appropriate flow rate, temperature, and shear force for space animal cell culture, a closed-loop microfluidic circuit and proportional, integrating, and differentiation (PID) algorithm are employed.
This paper also illustrates system architecture and operating method of the principle prototype.
The dynamic culture, autofocus observation, and recording of M763 cells are performed successfully within 72 h in the laboratory environment.
This research can provide a reference for space flight mission that carries an apparatus with similar functions.
American Psychological Association (APA)
Fan, Shangchun& Sun, Jinhao& Xing, Weiwei& Li, Cheng& Wang, Dongxue. 2013. Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling. Journal of Applied Mathematics،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-467299
Modern Language Association (MLA)
Fan, Shangchun…[et al.]. Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling. Journal of Applied Mathematics No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-467299
American Medical Association (AMA)
Fan, Shangchun& Sun, Jinhao& Xing, Weiwei& Li, Cheng& Wang, Dongxue. Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling. Journal of Applied Mathematics. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-467299
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467299