Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct
Joint Authors
Wang, Jinmei
You, X. Joann
Klassen, Henry
Yang, Jing
Song, Tianran
Gu, Ping
Liew, Chee Gee
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Work has shown that stem cell transplantation can rescue or replace neurons in models of retinal degenerative disease.
Neural progenitor cells (NPCs) modified to overexpress neurotrophic factors are one means of providing sustained delivery of therapeutic gene products in vivo.
To develop a nonrodent animal model of this therapeutic strategy, we previously derived NPCs from the fetal cat brain (cNPCs).
Here we use bicistronic feline lentiviral vectors to transduce cNPCs with glial cell-derived neurotrophic factor (GDNF) together with a GFP reporter gene.
Transduction efficacy is assessed, together with transgene expression level and stability during induction of cellular differentiation, together with the influence of GDNF transduction on growth and gene expression profile.
We show that GDNF overexpressing cNPCs expand in vitro, coexpress GFP, and secrete high levels of GDNF protein—before and after differentiation—all qualities advantageous for use as a cell-based approach in feline models of neural degenerative disease.
American Psychological Association (APA)
You, X. Joann& Gu, Ping& Wang, Jinmei& Song, Tianran& Yang, Jing& Liew, Chee Gee…[et al.]. 2010. Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct. Journal of Ophthalmology،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-467363
Modern Language Association (MLA)
You, X. Joann…[et al.]. Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct. Journal of Ophthalmology No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-467363
American Medical Association (AMA)
You, X. Joann& Gu, Ping& Wang, Jinmei& Song, Tianran& Yang, Jing& Liew, Chee Gee…[et al.]. Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct. Journal of Ophthalmology. 2010. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-467363
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467363