Angiotensin I-Converting Enzyme Inhibitor Derived from Cross-Linked Oyster Protein
Joint Authors
Choi, Yeung-Joon
Choung, Se-Young
Ha, Jong-Myung
Kim, Jin-Soo
Xie, Cheng-Liang
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Following cross-linking by microbial transglutaminase, modified oyster proteins were hydrolyzed to improve inhibitory activity against angiotensin-converting enzyme (ACE) inhibitory activity with the use of a single protease, or a combination of six proteases.
The oyster hydrolysate with the lowest 50% ACE inhibitory concentration (IC50) of 0.40 mg/mL was obtained by two-step hydrolysis of the cross-linked oyster protein using Protamex and Neutrase.
Five ACE inhibitory peptides were purified from the oyster hydrolysate using a multistep chromatographic procedure comprised of ion-exchange, size exclusion, and reversed-phase liquid chromatography.
Their sequences were identified as TAY, VK, KY, FYN, and YA, using automated Edman degradation and mass spectrometry.
These peptides were synthesized, and their IC50 values were measured to be 16.7, 29.0, 51.5, 68.2, and 93.9 μM, respectively.
Toxicity of the peptides on the HepG2 cell line was not detected.
The oyster hydrolysate also significantly decreased the systolic blood pressure of spontaneously hypertensive rats (SHR).
The antihypertensive effect of the oyster hydrolysate on SHR was rapid and long-lasting, compared to commercially obtained sardine hydrolysate.
These results suggest that the oyster hydrolysate could be a source of effective nutraceuticals against hypertension.
American Psychological Association (APA)
Xie, Cheng-Liang& Kim, Jin-Soo& Ha, Jong-Myung& Choung, Se-Young& Choi, Yeung-Joon. 2014. Angiotensin I-Converting Enzyme Inhibitor Derived from Cross-Linked Oyster Protein. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-467386
Modern Language Association (MLA)
Xie, Cheng-Liang…[et al.]. Angiotensin I-Converting Enzyme Inhibitor Derived from Cross-Linked Oyster Protein. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-467386
American Medical Association (AMA)
Xie, Cheng-Liang& Kim, Jin-Soo& Ha, Jong-Myung& Choung, Se-Young& Choi, Yeung-Joon. Angiotensin I-Converting Enzyme Inhibitor Derived from Cross-Linked Oyster Protein. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-467386
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467386