Shikonin Suppresses Human T Lymphocyte Activation through Inhibition of IKKβ Activity and JNK Phosphorylation
Joint Authors
Yan, Fenggen
Liu, Liang
Li, Ting
Zhou, Hua
Wang, Rui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The key role of T cells has been elaborated in mediating immune responses and pathogenesis of human inflammatory and autoimmune conditions.
In the current study the effect of shikonin, a compound isolated from a medicinal plant, on inhibition of T-cell activation was firstly examined by using primary human T lymphocytes isolated from buffy coat.
Results showed that shikonin dose dependently suppressed T-cell proliferation, IL-2 and IFN-γ secretion, CD69 and CD25 expression, as well as cell cycle arrest activated by costimulation of PMA/ionomycin or OKT-3/CD28 monoclonal antibodies.
Moreover, these inhibitory responses mediated by shikonin were found to be associated with suppression of the NF-κB signaling pathway via inhibition of the IKKα/β phosphorylation, IκB-α phosphorylation and degradation, and NF-κB nuclear translocation by directly decreasing IKKβ activity.
Moreover, shikonin suppressed JNK phosphorylation in the MAPKs pathway of T cells.
In this connection, we conclude that shikonin could suppress T lymphocyte activation through suppressing IKKβ activity and JNK signaling, which suggests that shikonin is valuable for further investigation as a potential immunosuppressive agent.
American Psychological Association (APA)
Li, Ting& Yan, Fenggen& Wang, Rui& Zhou, Hua& Liu, Liang. 2013. Shikonin Suppresses Human T Lymphocyte Activation through Inhibition of IKKβ Activity and JNK Phosphorylation. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-467418
Modern Language Association (MLA)
Li, Ting…[et al.]. Shikonin Suppresses Human T Lymphocyte Activation through Inhibition of IKKβ Activity and JNK Phosphorylation. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-467418
American Medical Association (AMA)
Li, Ting& Yan, Fenggen& Wang, Rui& Zhou, Hua& Liu, Liang. Shikonin Suppresses Human T Lymphocyte Activation through Inhibition of IKKβ Activity and JNK Phosphorylation. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-467418
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467418