Role of Calcium in Phosphatidylserine Externalisation in Red Blood Cells from Sickle Cell Patients
Joint Authors
Rees, David Charles
Weiss, Erwin
Gibson, John Stanley
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Phosphatidylserine exposure occurs in red blood cells (RBCs) from sickle cell disease (SCD) patients and is increased by deoxygenation.
The mechanisms responsible remain unclear.
RBCs from SCD patients also have elevated cation permeability, and, in particular, a deoxygenation-induced cation conductance which mediates Ca2+ entry, providing an obvious link with phosphatidylserine exposure.
The role of Ca2+ was investigated using FITC-labelled annexin.
Results confirmed high phosphatidylserine exposure in RBCs from SCD patients increasing upon deoxygenation.
When deoxygenated, phosphatidylserine exposure was further elevated as extracellular [Ca2+] was increased.
This effect was inhibited by dipyridamole, intracellular Ca2+ chelation, and Gardos channel inhibition.
Phosphatidylserine exposure was reduced in high K+ saline.
Ca2+ levels required to elicit phosphatidylserine exposure were in the low micromolar range.
Findings are consistent with Ca2+ entry through the deoxygenation-induced pathway (Psickle), activating the Gardos channel.
[Ca2+] required for phosphatidylserine scrambling are in the range achievable in vivo.
American Psychological Association (APA)
Weiss, Erwin& Rees, David Charles& Gibson, John Stanley. 2010. Role of Calcium in Phosphatidylserine Externalisation in Red Blood Cells from Sickle Cell Patients. Anemia،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-467456
Modern Language Association (MLA)
Weiss, Erwin…[et al.]. Role of Calcium in Phosphatidylserine Externalisation in Red Blood Cells from Sickle Cell Patients. Anemia No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-467456
American Medical Association (AMA)
Weiss, Erwin& Rees, David Charles& Gibson, John Stanley. Role of Calcium in Phosphatidylserine Externalisation in Red Blood Cells from Sickle Cell Patients. Anemia. 2010. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-467456
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467456