Effective and Steady Differentiation of a Clonal Derivative of P19CL6 Embryonal Carcinoma Cell Line into Beating Cardiomyocytes
Joint Authors
Nakajima, Takayuki
Ogawa, Kazushige
Ishii, Maki
Mueller, Itsuki
Kobayashi, Ryosuke
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The P19CL6 cell line is a useful model to study cardiac differentiation in vitro.
However, large variations were noticed in the differentiation rates among previous reports as well as our individual experiments.
To overcome the unstable differentiation, we established P19CL6-A1, a new clonal derivative of P19CL6 that could differentiate into cardiomyocytes more efficiently and stably than the parent using the double stimulation with 5-Aza and DMSO based on the previous report.
We also introduced a new software, Visorhythm, that can analyze the temporal variations in the beating rhythms and can chart correlograms displaying the oscillated rhythms.
Using P19CL6-A1-derived cardiomyocytes and the software, we demonstrated that the correlograms could clearly display the enhancement of beating rates by cardiotonic reagents.
These indicate that a combination of P19CL6-A1 and Visorhythm is a useful tool that can provide invaluable assistance in inotropic drug discovery, drug screening, and toxicity testing.
American Psychological Association (APA)
Mueller, Itsuki& Kobayashi, Ryosuke& Nakajima, Takayuki& Ishii, Maki& Ogawa, Kazushige. 2010. Effective and Steady Differentiation of a Clonal Derivative of P19CL6 Embryonal Carcinoma Cell Line into Beating Cardiomyocytes. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-467496
Modern Language Association (MLA)
Mueller, Itsuki…[et al.]. Effective and Steady Differentiation of a Clonal Derivative of P19CL6 Embryonal Carcinoma Cell Line into Beating Cardiomyocytes. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-467496
American Medical Association (AMA)
Mueller, Itsuki& Kobayashi, Ryosuke& Nakajima, Takayuki& Ishii, Maki& Ogawa, Kazushige. Effective and Steady Differentiation of a Clonal Derivative of P19CL6 Embryonal Carcinoma Cell Line into Beating Cardiomyocytes. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-467496
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467496