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Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons
Joint Authors
Nakamura, Masanori
Ujihara, Yoshihiro
Miyazaki, Hiroshi
Wada, Shigeo
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We developed a method to reconstruct cell geometry from confocal fluorescence microscopy images of the cytoskeleton.
In the method, region growing was implemented twice.
First, it was applied to the extracellular regions to differentiate them from intracellular noncytoskeletal regions, which both appear black in fluorescence microscopy imagery, and then to cell regions for cell identification.
Analysis of morphological parameters revealed significant changes in cell shape associated with cytoskeleton disruption, which offered insight into the mechanical role of the cytoskeleton in maintaining cell shape.
The proposed segmentation method is promising for investigations on cell morphological changes with respect to internal cytoskeletal structures.
American Psychological Association (APA)
Ujihara, Yoshihiro& Nakamura, Masanori& Miyazaki, Hiroshi& Wada, Shigeo. 2013. Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons. Computational and Mathematical Methods in Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-467566
Modern Language Association (MLA)
Ujihara, Yoshihiro…[et al.]. Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons. Computational and Mathematical Methods in Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-467566
American Medical Association (AMA)
Ujihara, Yoshihiro& Nakamura, Masanori& Miyazaki, Hiroshi& Wada, Shigeo. Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons. Computational and Mathematical Methods in Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-467566
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467566