Investigating hsp Gene Expression in Liver of Channa striatus under Heat Stress for Understanding the Upper Thermal Acclimation
Joint Authors
Suar, Mrutyunjay
Purohit, Gopal Krishna
Mahanty, Arabinda
Mohanty, Sasmita
Mohanty, Bimal Prasanna
Sharma, Anil Prakash
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Changes in hsp gene expression profiles in murrel Channa striatus experimentally exposed to temperature stress (36°C) for 4, 15, and 30 days were investigated; fish collected from aquaculture ponds and maintained in laboratory at the pond temperature (25 ± 1°C) served as control.
Channa collected from a hot spring runoff (36°C) was included in the study to examine the hsp profiles beyond 30 days of exposure.
Gene expression analyses of a battery of hsps in liver tissues were carried out by quantitative RT-PCR and protein expressions were analyzed by immunoblotting.
hsps could be grouped into three clusters based on similarity in response to heat stress: hsp70, hsp78, and hsp60, whose transcript level continued to increase with duration of exposure; hsp90 and hsp110 that increased to a much higher level and then decreased; hsp27 and hsp47 that did not significantly vary as compared to control.
The results suggest that Hsp70, Hsp78, and Hsp60 are involved in thermal acclimation and long term survival at high temperature.
Fish living in the hot spring runoff appears to continuously express hsps that can be approximated by long term induction of hsps in farmed fish if temperature of their environment is raised to 36°C.
American Psychological Association (APA)
Purohit, Gopal Krishna& Mahanty, Arabinda& Suar, Mrutyunjay& Sharma, Anil Prakash& Mohanty, Bimal Prasanna& Mohanty, Sasmita. 2014. Investigating hsp Gene Expression in Liver of Channa striatus under Heat Stress for Understanding the Upper Thermal Acclimation. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-467601
Modern Language Association (MLA)
Purohit, Gopal Krishna…[et al.]. Investigating hsp Gene Expression in Liver of Channa striatus under Heat Stress for Understanding the Upper Thermal Acclimation. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-467601
American Medical Association (AMA)
Purohit, Gopal Krishna& Mahanty, Arabinda& Suar, Mrutyunjay& Sharma, Anil Prakash& Mohanty, Bimal Prasanna& Mohanty, Sasmita. Investigating hsp Gene Expression in Liver of Channa striatus under Heat Stress for Understanding the Upper Thermal Acclimation. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-467601
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467601