A Cell Model for Conditional Profiling of Androgen-Receptor-Interacting Proteins
Joint Authors
Hughes, I. A.
Davies, J. D.
Mooslehner, K. A.
Source
International Journal of Endocrinology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-27
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Partial androgen insensitivity syndrome (PAIS) is associated with impaired male genital development and can be transmitted through mutations in the androgen receptor (AR).
The aim of this study is to develop a cell model suitable for studying the impact AR mutations might have on AR interacting proteins.
For this purpose, male genital development relevant mouse cell lines were genetically modified to express a tagged version of wild-type AR, allowing copurification of multiprotein complexes under native conditions followed by mass spectrometry.
We report 57 known wild-type AR-interacting proteins identified in cells grown under proliferating and 65 under nonproliferating conditions.
Of those, 47 were common to both samples suggesting different AR protein complex components in proliferating and proliferation-inhibited cells from the mouse proximal caput epididymus.
These preliminary results now allow future studies to focus on replacing wild-type AR with mutant AR to uncover differences in protein interactions caused by AR mutations involved in PAIS.
American Psychological Association (APA)
Mooslehner, K. A.& Davies, J. D.& Hughes, I. A.. 2012. A Cell Model for Conditional Profiling of Androgen-Receptor-Interacting Proteins. International Journal of Endocrinology،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-467615
Modern Language Association (MLA)
Mooslehner, K. A.…[et al.]. A Cell Model for Conditional Profiling of Androgen-Receptor-Interacting Proteins. International Journal of Endocrinology No. 2012 (2012), pp.1-15.
https://search.emarefa.net/detail/BIM-467615
American Medical Association (AMA)
Mooslehner, K. A.& Davies, J. D.& Hughes, I. A.. A Cell Model for Conditional Profiling of Androgen-Receptor-Interacting Proteins. International Journal of Endocrinology. 2012. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-467615
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467615