Low Level of Her-2 Locus Amplification by Fluorescent In Situ Hybridization Does Not Correlate with Her-2 Protein Overexpression by Immunohistochemistry in Barrett's Esophagus
Joint Authors
Milano, Francesca
Krishnadath, Kausillia K.
Rygiel, Agnieszka M.
ten Kate, Fiebo J.
Bergman, Jacques J. G. H. M.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
An accurate evaluation of the Her-2 status has important prognostic and therapeutic implications in many carcinomas.
The aim of the study was to correlate Her-2 locus (17q11.2) amplification and chromosome 17 gains as assessed by fluorescent in situ hybridization (FISH) with Her-2 protein overexpression by immunohistochemistry (IHC) in patients with Barrett's esophagus (BE) and esophageal adenocarcinoma (EAC).
We analyzed 34 patients with Her-2 amplification and/or chromosome 17gains using FISH on brush cytology specimens.
Seven patients (21%) showed high Her-2 locus amplification (Her-2: Cep17 ≥ 5 : 1), 5 (15%) showed low Her-2 locus amplification (Her-2: Cep17 ≥ 2 < 5 : 1), and 22 (65%) displayed gains of chromosome 17 only.
Further, we confirmed Her-2 amplification on corresponding biopsies that were taken at the same occasion as the cytologybrushings.
Then, we compared the FISH results with IHC data obtained from the corresponding biopsies and showed that low level of Her-2 amplification does not correlate with Her-2 protein overexpression (score +3/+2; P=1), in contrast to the high amplification level (P=.001).
Thus, in our population of BE and EAC patients, low level of Her-2 amplification does not result in detectable level of Her-2 protein as assessed by IHC.
American Psychological Association (APA)
Rygiel, Agnieszka M.& Milano, Francesca& ten Kate, Fiebo J.& Bergman, Jacques J. G. H. M.& Krishnadath, Kausillia K.. 2010. Low Level of Her-2 Locus Amplification by Fluorescent In Situ Hybridization Does Not Correlate with Her-2 Protein Overexpression by Immunohistochemistry in Barrett's Esophagus. Journal of Oncology،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-467685
Modern Language Association (MLA)
Rygiel, Agnieszka M.…[et al.]. Low Level of Her-2 Locus Amplification by Fluorescent In Situ Hybridization Does Not Correlate with Her-2 Protein Overexpression by Immunohistochemistry in Barrett's Esophagus. Journal of Oncology No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-467685
American Medical Association (AMA)
Rygiel, Agnieszka M.& Milano, Francesca& ten Kate, Fiebo J.& Bergman, Jacques J. G. H. M.& Krishnadath, Kausillia K.. Low Level of Her-2 Locus Amplification by Fluorescent In Situ Hybridization Does Not Correlate with Her-2 Protein Overexpression by Immunohistochemistry in Barrett's Esophagus. Journal of Oncology. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-467685
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467685