Synergistic Effect of MiR-146a Mimic and Cetuximab on Hepatocellular Carcinoma Cells
Joint Authors
Huang, Suning
He, Rongquan
Dang, Yiwu
Rong, Minhua
Chen, Gang
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-07
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Previously, we found that the expression of microRNA-146a (miR-146a) was downregulated in hepatocellular carcinoma (HCC) formalin-fixed paraffin-embedded (FFPE) tissues compared to the adjacent noncancerous hepatic tissues.
In the current study, we have explored the in vitro effect of miR-146a on the malignant phenotypes of HCC cells.
MiR-146a mimic could suppress cell growth and increase cellular apoptosis in HCC cell lines HepG2, HepB3, and SNU449, as assessed by spectrophotometry, fluorimetry, and fluorescence microscopy, respectively.
Furthermore, western blot showed that miR-146a mimic downregulated EGFR, ERK1/2, and stat5 signalings.
These effects were less potent compared to that of a siRNA targeting EGFR, a known target gene of miR-146a.
Moreover, miR-146a mimic could enhance the cell growth inhibition and apoptosis induction impact of various EGFR targeting agents.
The most potent combination was miR-146a mimic with cetuximab, presenting a synergistic effect.
In conclusion, miR-146a plays a vital role in the cell growth and apoptosis of HCC cells and inducing miR-146a level might be a critical targeted molecular therapy strategy for HCC.
American Psychological Association (APA)
Huang, Suning& He, Rongquan& Rong, Minhua& Dang, Yiwu& Chen, Gang. 2014. Synergistic Effect of MiR-146a Mimic and Cetuximab on Hepatocellular Carcinoma Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-467815
Modern Language Association (MLA)
Huang, Suning…[et al.]. Synergistic Effect of MiR-146a Mimic and Cetuximab on Hepatocellular Carcinoma Cells. BioMed Research International No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-467815
American Medical Association (AMA)
Huang, Suning& He, Rongquan& Rong, Minhua& Dang, Yiwu& Chen, Gang. Synergistic Effect of MiR-146a Mimic and Cetuximab on Hepatocellular Carcinoma Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-467815
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467815