Monocytes Do Not Transdifferentiate into Proper Osteoblasts
Joint Authors
Buschner, Peter
Schyschka, Lilianna
Schmitt, Andreas
Kühnl, Andreas
Döbele, Stefan
Siebenlist, Sebastian
Lucke, Martin
Stöckle, Ulrich
Nussler, Andreas K.
Ehnert, Sabrina
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Recent publications suggested that monocytes might be an attractive cell type to transdifferentiate into various cellular phenotypes.
Aim was, therefore, to evaluate the potential of blood monocytes to transdifferentiate into osteoblasts.
Monocytes isolated from peripheral blood were subjected to two previously published treatments to obtain unique, multipotent cell fractions, named programmable cells of monocytic origin (PCMOs) and monocyte-derived mesenchymal progenitor cells (MOMPs).
Subsequently, MOMPs and PCMOs were treated with osteogenic differentiation medium (including either vitamin D or dexamethasone) for 14 days.
Regarding a variety of surface markers, no differences between MOMPs, PCMOs, and primary monocytes could be detected.
The treatment with osteogenic medium neither resulted in loss of hematopoietic markers nor in adoption of mesenchymal phenotype in all cell types.
No significant effect was observed regarding the expression of osteogenic transcription factors, bone-related genes, or production of mineralized matrix.
Osteogenic medium resulted in activation of monocytes and appearance of osteoclasts.
In conclusion, none of the investigated monocyte cell types showed any transdifferentiation characteristics under the tested circumstances.
Based on our data, we rather see an activation and maturation of monocytes towards macrophages and osteoclasts.
American Psychological Association (APA)
Schmitt, Andreas& Ehnert, Sabrina& Schyschka, Lilianna& Buschner, Peter& Kühnl, Andreas& Döbele, Stefan…[et al.]. 2012. Monocytes Do Not Transdifferentiate into Proper Osteoblasts. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-467888
Modern Language Association (MLA)
Schmitt, Andreas…[et al.]. Monocytes Do Not Transdifferentiate into Proper Osteoblasts. The Scientific World Journal No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-467888
American Medical Association (AMA)
Schmitt, Andreas& Ehnert, Sabrina& Schyschka, Lilianna& Buschner, Peter& Kühnl, Andreas& Döbele, Stefan…[et al.]. Monocytes Do Not Transdifferentiate into Proper Osteoblasts. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-467888
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467888