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Quantitative Determination of Flavonoids and Chlorogenic Acid in the Leaves of Arbutus unedo L. Using Thin Layer Chromatography
Joint Authors
Maleš, Željan
Bojić, Mirza
Šarić, Darija
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-31
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
The plant species Arbutus unedo shows numerous beneficial pharmacological effects (antiseptic, antidiabetic, antidiarrheal, astringent, depurative, antioxidant, antihypertensive, antithrombotic, and anti-inflammatory).
For the medicinal use, standardization of extracts is a necessity, as different compounds are responsible for different biological activities.
In this paper, we analyze monthly changes in the content of quercitrin, isoquercitrin, hyperoside, and chlorogenic acid.
Methanolic extracts of the leaves are analyzed by HPTLC for the identification and quantification of individual polyphenol, and DPPH test is used to determine antioxidant activity.
Based on the results obtained, the leaves should be collected in January to obtain the highest concentrations of hyperoside and quercitrin (0.35 mg/g and 1.94 mg/g, resp.), in June, July, and October for chlorogenic acid (1.45–1.46 mg/g), and for the fraction of quercitrin and isoquercitrin in November (1.98 mg/g and 0.33 mg/g, resp.).
Optimal months for the collection of leaves with the maximum recovery of individual polyphenol suggested in this work could direct the pharmacological usage of the polyvalent herbal drugs.
American Psychological Association (APA)
Maleš, Željan& Šarić, Darija& Bojić, Mirza. 2013. Quantitative Determination of Flavonoids and Chlorogenic Acid in the Leaves of Arbutus unedo L. Using Thin Layer Chromatography. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-467939
Modern Language Association (MLA)
Maleš, Željan…[et al.]. Quantitative Determination of Flavonoids and Chlorogenic Acid in the Leaves of Arbutus unedo L. Using Thin Layer Chromatography. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-467939
American Medical Association (AMA)
Maleš, Željan& Šarić, Darija& Bojić, Mirza. Quantitative Determination of Flavonoids and Chlorogenic Acid in the Leaves of Arbutus unedo L. Using Thin Layer Chromatography. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-467939
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467939