Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines
Joint Authors
Garrido-Arandia, M.
Canto, G.
Pacios, L. F.
Díaz-Perales, A.
Tordesillas, L.
Cuesta-Herranz, J.
Gómez-Casado, C.
Varela, J.
Blanca-López, N.
Gamboa, P.
Source
Clinical and Developmental Immunology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-14
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Nowadays, treatment of food allergy only considered the avoidance of the specific food.
However, the possibility of cross-reactivity makes this practice not very effective.
Immunotherapy may exhibit as a good alternative to food allergy treatment.
The use of hypoallergenic molecules with reduced IgE binding capacity but with ability to stimulate the immune system is a promising tool which could be developed for immunotherapy.
In this study, three mutants of Pru p 3, the principal allergen of peach, were produced based on the described mimotope and T cell epitopes, by changing the specific residues to alanine, named as Pru p 3.01, Pru p 3.02, and Pru p 3.03.
Pru p 3.01 showed very similar allergenic activity as the wild type by in vitro assays.
However, Pru p 3.02 and Pru p 3.03 presented reduced IgE binding with respect to the native form, by in vitro, ex vivo, and in vivo assays.
In addition, Pru p 3.03 had affected the IgG4 binding capacity and presented a random circular dichroism, which was reflected in the nonrecognition by specific antibodies anti-Pru p 3.
Nevertheless, both Pru p 3.02 and Pru p 3.03 maintained the binding to IgG1 and their ability to activate T lymphocytes.
Thus, Pru p 3.02 and Pru p 3.03 could be good candidates for potential immunotherapy in peach-allergic patients.
American Psychological Association (APA)
Gómez-Casado, C.& Garrido-Arandia, M.& Gamboa, P.& Blanca-López, N.& Canto, G.& Varela, J.…[et al.]. 2013. Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-467943
Modern Language Association (MLA)
Gómez-Casado, C.…[et al.]. Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines. Clinical and Developmental Immunology No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-467943
American Medical Association (AMA)
Gómez-Casado, C.& Garrido-Arandia, M.& Gamboa, P.& Blanca-López, N.& Canto, G.& Varela, J.…[et al.]. Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-467943
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467943