Combined Reversed Phase HPLC, Mass Spectrometry, and NMR Spectroscopy for a Fast Separation and Efficient Identification of Phosphatidylcholines
Joint Authors
Thiele, Herbert
Willmann, Jan
Leibfritz, Dieter
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In respect of the manifold involvement of lipids in biochemical processes, the analysis of intact and underivatised lipids of body fluids as well as cell and tissue extracts is still a challenging task, if detailed molecular information is required.
Therefore, the advantage of combined use of high-pressure liquid chromatography (HPLC), mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy will be shown analyzing three different types of extracts of the ubiquitous membrane component phosphatidylcholine.
At first, different reversed phase modifications were tested on phosphatidylcholines (PC) with the same effective carbon number (ECN) for their applicability in lipid analysis.
The results were taken to improve the separation of three natural PC extract types and a new reversed phase (RP)-HPLC method was developed.
The individual species were characterized by one- and two-dimensional NMR and positive or negative ion mode quadrupole time of flight (q-TOF)-MS as well as MS/MS techniques.
Furthermore, ion suppression effects during electrospray ionisation (ESI), difficulties, limits, and advantages of the individual analytical techniques are addressed.
American Psychological Association (APA)
Willmann, Jan& Thiele, Herbert& Leibfritz, Dieter. 2010. Combined Reversed Phase HPLC, Mass Spectrometry, and NMR Spectroscopy for a Fast Separation and Efficient Identification of Phosphatidylcholines. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-467960
Modern Language Association (MLA)
Willmann, Jan…[et al.]. Combined Reversed Phase HPLC, Mass Spectrometry, and NMR Spectroscopy for a Fast Separation and Efficient Identification of Phosphatidylcholines. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-467960
American Medical Association (AMA)
Willmann, Jan& Thiele, Herbert& Leibfritz, Dieter. Combined Reversed Phase HPLC, Mass Spectrometry, and NMR Spectroscopy for a Fast Separation and Efficient Identification of Phosphatidylcholines. Journal of Biomedicine and Biotechnology. 2010. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-467960
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-467960