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Both Basic and Acidic Amino Acid Residues of IpTxa Are Involved in Triggering Substate of RyR1
Joint Authors
Seo, In Ra
Song, Dong Woo
Kim, Do Han
Kang, Dae Eun
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Imperatoxin A (IpTxa) is known to modify the gating of skeletal ryanodine receptor (RyR1).
In this paper, the ability of charged aa residues of IpTxa to induce substate of native RyR1 in HSR was examined.
Our results show that the basic residues (e.g., Lys19, Lys20, Lys22, Arg23, and Arg24) are important for producing substate of RyR1.
In addition, other basic residues (e.g., Lys30, Arg31, and Arg33) near the C-terminus and some acidic residues (e.g., Glu29, Asp13, and Asp2) are also involved in the generation of substate.
Residues such as Lys8 and Thr26 may be involved in the self-regulation of substate of RyR1, since alanine substitution of the aa residues led to a drastic conversion to the substate.
The modifications of the channel gating by the wild-type and mutant toxins were similar in purified RyR1.
Taken together, the specific charge distributions on the surface of IpTxa are essential for regulation of the channel gating of RyR1.
American Psychological Association (APA)
Seo, In Ra& Kang, Dae Eun& Song, Dong Woo& Kim, Do Han. 2011. Both Basic and Acidic Amino Acid Residues of IpTxa Are Involved in Triggering Substate of RyR1. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-468024
Modern Language Association (MLA)
Seo, In Ra…[et al.]. Both Basic and Acidic Amino Acid Residues of IpTxa Are Involved in Triggering Substate of RyR1. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-468024
American Medical Association (AMA)
Seo, In Ra& Kang, Dae Eun& Song, Dong Woo& Kim, Do Han. Both Basic and Acidic Amino Acid Residues of IpTxa Are Involved in Triggering Substate of RyR1. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-468024
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468024