Preparation of Laccase Immobilized Cryogels and Usage for Decolorization
Author
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Poly(methyl methacrylate-co-glycidyl methacrylate) (poly(MMA-co-GMA)) cryogels were synthesized by radical cryopolymerization technique.
Then, laccase enzyme was covalently attached to the cryogel and characterized by using swelling studies and SEM and EDX analyses.
Kinetic properties and optimum conditions of the immobilized and free laccase were studied and it was found that Vmax of the immobilized laccase was lower than that of free laccase.
Km of the immobilized laccase was increased upon immobilization.
Optimum pH was found to be 4.0 for each type of laccase, while optimum temperature was shifted to the warmer region after the immobilization.
It was also found that thermal stability of the immobilized laccase was higher than that of free laccase.
Immobilized laccase could be used for 10 times successive reuse with no significant decrease in its activity.
Also, these laccase immobilized cryogels were successfully used for the decolorization of seven different dyes.
American Psychological Association (APA)
Uygun, Murat. 2013. Preparation of Laccase Immobilized Cryogels and Usage for Decolorization. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-468119
Modern Language Association (MLA)
Uygun, Murat. Preparation of Laccase Immobilized Cryogels and Usage for Decolorization. Journal of Chemistry No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-468119
American Medical Association (AMA)
Uygun, Murat. Preparation of Laccase Immobilized Cryogels and Usage for Decolorization. Journal of Chemistry. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-468119
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468119