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Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC
Joint Authors
Neuss, Sabine
Adamzyk, Carina
Emonds, Tanja
Falkenstein, Julia
Lethaus, Bernd
Tolba, René H.
Jahnen-Dechent, Wilhelm
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-21
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Orthopedic implants including engineered bone tissue are commonly tested in sheep.
To avoid rejection of heterologous or xenogeneic cells, autologous cells are preferably used, that is, ovine mesenchymal stem cells (oMSC).
Unlike human MSC, ovine MSC are not well studied regarding isolation, expansion, and characterization.
Here we investigated the impact of culture media composition on growth characteristics, differentiation, and surface antigen expression of oMSC.
The culture media varied in fetal calf serum (FCS) content and in the addition of supplements and/or additional epidermal growth factor (EGF).
We found that FCS strongly influenced oMSC proliferation and that specific combinations of supplemental factors (MCDB-201, ITS-plus, dexamethasone, and L-ascorbic acid) determined the expression of surface epitopes.
We compared two published protocols for oMSC differentiation towards the osteogenic, adipogenic, and chondrogenic fate and found (i) considerable donor to donor variations, (ii) protocol-dependent variations, and (iii) variations resulting from the preculture medium composition.
Our results indicate that the isolation and culture of oMSC in different growth media are highly variable regarding oMSC phenotype and behaviour.
Furthermore, variations from donor to donor critically influence growth rate, surface marker expression, and differentiation.
American Psychological Association (APA)
Adamzyk, Carina& Emonds, Tanja& Falkenstein, Julia& Tolba, René H.& Jahnen-Dechent, Wilhelm& Lethaus, Bernd…[et al.]. 2013. Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC. Stem Cells International،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-468133
Modern Language Association (MLA)
Adamzyk, Carina…[et al.]. Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC. Stem Cells International No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-468133
American Medical Association (AMA)
Adamzyk, Carina& Emonds, Tanja& Falkenstein, Julia& Tolba, René H.& Jahnen-Dechent, Wilhelm& Lethaus, Bernd…[et al.]. Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC. Stem Cells International. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-468133
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468133