Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-Induced γH2A.X Foci
Joint Authors
Xu, Zongli
Taylor, Jack A.
Markunas, Christina A.
Umbach, David M.
Source
Journal of Cancer Epidemiology
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-03-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Nonsynonymous SNPs (nsSNPs) in DNA repair genes may be important determinants of DNA damage and cancer risk.
We applied a set of screening criteria to a large number of nsSNPs and selected a subset of SNPs that were likely candidates for phenotypic effects on DNA double-strand break repair (DSBR).
In order to induce and follow DSBR, we exposed panels of cell lines to gamma irradiation and followed the formation and disappearance of γH2A.X foci over time.
All panels of cell lines showed significant increases in number, intensity, and area of foci at both the 1-hour and 3-hour time points.
Twenty four hours following exposure, the number of foci returned to preexposure levels in all cell lines, whereas the size and intensity of foci remained significantly elevated.
We saw no significant difference in γH2A.X foci between controls and any of the panels of cell lines representing the different nsSNPs.
American Psychological Association (APA)
Markunas, Christina A.& Umbach, David M.& Xu, Zongli& Taylor, Jack A.. 2009. Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-Induced γH2A.X Foci. Journal of Cancer Epidemiology،Vol. 2008, no. 2008, pp.1-8.
https://search.emarefa.net/detail/BIM-468142
Modern Language Association (MLA)
Markunas, Christina A.…[et al.]. Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-Induced γH2A.X Foci. Journal of Cancer Epidemiology No. 2008 (2008), pp.1-8.
https://search.emarefa.net/detail/BIM-468142
American Medical Association (AMA)
Markunas, Christina A.& Umbach, David M.& Xu, Zongli& Taylor, Jack A.. Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-Induced γH2A.X Foci. Journal of Cancer Epidemiology. 2009. Vol. 2008, no. 2008, pp.1-8.
https://search.emarefa.net/detail/BIM-468142
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468142