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Sequence-Based Appraisal of the Genes Encoding Neck and Carbohydrate Recognition Domain of Conglutinin in Blackbuck (Antilope cervicapra) and Goat (Capra hircus)
Joint Authors
Doreswamy, Ramesh
Das, Asit
Saini, Mohini
Sharma, Anil K.
Siddappa, Chandra Mohan
Barik, Sasmita
Gupta, Praveen K.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Conglutinin, a collagenous C-type lectin, acts as soluble pattern recognition receptor (PRR) in recognition of pathogens.
In the present study, genes encoding neck and carbohydrate recognition domain (NCRD) of conglutinin in goat and blackbuck were amplified, cloned, and sequenced.
The obtained 488 bp ORFs encoding NCRD were submitted to NCBI with accession numbers KC505182 and KC505183.
Both nucleotide and predicted amino acid sequences were analysed with sequences of other ruminants retrieved from NCBI GenBank using DNAstar and Megalign5.2 software.
Sequence analysis revealed maximum similarity of blackbuck sequence with wild ruminants like nilgai and buffalo, whereas goat sequence displayed maximum similarity with sheep sequence at both nucleotide and amino acid level.
Phylogenetic analysis further indicated clear divergence of wild ruminants from the domestic ruminants in separate clusters.
The predicted secondary structures of NCRD protein in goat and blackbuck using SWISSMODEL ProtParam online software were found to possess 6 beta-sheets and 3 alpha-helices which are identical to the result obtained in case of sheep, cattle, buffalo, and nilgai.
However, quaternary structure in goat, sheep, and cattle was found to differ from that of buffalo, nilgai, and blackbuck, suggesting a probable variation in the efficiency of antimicrobial activity among wild and domestic ruminants.
American Psychological Association (APA)
Barik, Sasmita& Siddappa, Chandra Mohan& Saini, Mohini& Doreswamy, Ramesh& Das, Asit& Sharma, Anil K.…[et al.]. 2014. Sequence-Based Appraisal of the Genes Encoding Neck and Carbohydrate Recognition Domain of Conglutinin in Blackbuck (Antilope cervicapra) and Goat (Capra hircus). BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-468202
Modern Language Association (MLA)
Barik, Sasmita…[et al.]. Sequence-Based Appraisal of the Genes Encoding Neck and Carbohydrate Recognition Domain of Conglutinin in Blackbuck (Antilope cervicapra) and Goat (Capra hircus). BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-468202
American Medical Association (AMA)
Barik, Sasmita& Siddappa, Chandra Mohan& Saini, Mohini& Doreswamy, Ramesh& Das, Asit& Sharma, Anil K.…[et al.]. Sequence-Based Appraisal of the Genes Encoding Neck and Carbohydrate Recognition Domain of Conglutinin in Blackbuck (Antilope cervicapra) and Goat (Capra hircus). BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-468202
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468202