Live Cells as Dynamic Laboratories : Time Lapse Raman Spectral Microscopy of Nanoparticles with Both IgE Targeting and pH-Sensing Functions
Joint Authors
Nowak-Lovato, Kristy L.
Rector, Kirk D.
Source
International Journal of Analytical Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-24
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
This review captures the use of live cells as dynamic microlaboratories through implementation of labeled nanoparticles (nanosensors) that have both sensing and targeting functions.
The addition of 2,4-ε-dinitrophenol-L-lysine (DNP) as a FcεRI targeting ligand and 4-mercaptopyridine (4-MPy) as a pH-sensing ligand enables spatial and temporal monitoring of FcεRI receptors and their pH environment within the endocytic pathway.
To ensure reliability, the sensor is calibrated in vivo using the ionophore nigericin and standard buffer solutions to equilibrate the external [H+] concentration with that of the cell compartments.
This review highlights the nanosensors, ability to traffic and respond to pH of receptor-bound nanosensors (1) at physiological temperature (37°C) versus room temperature (25°C), (2) after pharmacological treatment with bafilomycin, an H+ ATPase pump inhibitor, or amiloride, an inhibitor of Na+/H+ exchange, and (3) in response to both temperature and pharmacological treatment.
Whole-cell, time lapse images are demonstrated to show the ability to transform live cells into dynamic laboratories to monitor temporal and spatial endosomal pH.
The versatility of these probes shows promise for future applications relevant to intracellular trafficking and intelligent drug design.
American Psychological Association (APA)
Nowak-Lovato, Kristy L.& Rector, Kirk D.. 2012. Live Cells as Dynamic Laboratories : Time Lapse Raman Spectral Microscopy of Nanoparticles with Both IgE Targeting and pH-Sensing Functions. International Journal of Analytical Chemistry،Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-468299
Modern Language Association (MLA)
Nowak-Lovato, Kristy L.& Rector, Kirk D.. Live Cells as Dynamic Laboratories : Time Lapse Raman Spectral Microscopy of Nanoparticles with Both IgE Targeting and pH-Sensing Functions. International Journal of Analytical Chemistry No. 2012 (2012), pp.1-16.
https://search.emarefa.net/detail/BIM-468299
American Medical Association (AMA)
Nowak-Lovato, Kristy L.& Rector, Kirk D.. Live Cells as Dynamic Laboratories : Time Lapse Raman Spectral Microscopy of Nanoparticles with Both IgE Targeting and pH-Sensing Functions. International Journal of Analytical Chemistry. 2012. Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-468299
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468299