Cultured Human Airway Epithelial Cells (Calu-3) : A Model of Human Respiratory Function, Structure, and Inflammatory Responses
Joint Authors
Chidekel, Aaron
Zhu, Yan
Shaffer, Thomas H.
Source
Critical Care Research and Practice
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
This article reviews the application of the human airway Calu-3 cell line as a respiratory model for studying the effects of gas concentrations, exposure time, biophysical stress, and biological agents on human airway epithelial cells.
Calu-3 cells are grown to confluence at an air-liquid interface on permeable supports.
To model human respiratory conditions and treatment modalities, monolayers are placed in an environmental chamber, and exposed to specific levels of oxygen or other therapeutic modalities such as positive pressure and medications to assess the effect of interventions on inflammatory mediators, immunologic proteins, and antibacterial outcomes.
Monolayer integrity and permeability and cell histology and viability also measure cellular response to therapeutic interventions.
Calu-3 cells exposed to graded oxygen concentrations demonstrate cell dysfunction and inflammation in a dose-dependent manner.
Modeling positive airway pressure reveals that pressure may exert a greater injurious effect and cytokine response than oxygen.
In experiments with pharmacological agents, Lucinactant is protective of Calu-3 cells compared with Beractant and control, and perfluorocarbons also protect against hyperoxia-induced airway epithelial cell injury.
The Calu-3 cell preparation is a sensitive and efficient preclinical model to study human respiratory processes and diseases related to oxygen- and ventilator-induced lung injury.
American Psychological Association (APA)
Zhu, Yan& Chidekel, Aaron& Shaffer, Thomas H.. 2010. Cultured Human Airway Epithelial Cells (Calu-3) : A Model of Human Respiratory Function, Structure, and Inflammatory Responses. Critical Care Research and Practice،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-468684
Modern Language Association (MLA)
Zhu, Yan…[et al.]. Cultured Human Airway Epithelial Cells (Calu-3) : A Model of Human Respiratory Function, Structure, and Inflammatory Responses. Critical Care Research and Practice No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-468684
American Medical Association (AMA)
Zhu, Yan& Chidekel, Aaron& Shaffer, Thomas H.. Cultured Human Airway Epithelial Cells (Calu-3) : A Model of Human Respiratory Function, Structure, and Inflammatory Responses. Critical Care Research and Practice. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-468684
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468684