Transcriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reduction
Joint Authors
Williams, Daniel
Hung, Chiu-Yueh
Xie, Jiahua
Kittur, Farooqahmed S.
Ibeanu, Gordon C.
Sun, Katherine H.
Tetteh, Antonia Y.
Source
International Journal of Microbiology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-15
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Bacteria can reduce toxic selenite into less toxic, elemental selenium (Se0), but the mechanism on how bacterial cells reduce selenite at molecular level is still not clear.
We used Escherichia coli strain K12, a common bacterial strain, as a model to study its growth response to sodium selenite (Na2SeO3) treatment and then used quantitative real-time PCR (qRT-PCR) to quantify transcript levels of three E.
coli selenopolypeptide genes and a set of machinery genes for selenocysteine (SeCys) biosynthesis and incorporation into polypeptides, whose involvements in the selenite reduction are largely unknown.
We determined that 5 mM Na2SeO3 treatment inhibited growth by ∼50% while 0.001 to 0.01 mM treatments stimulated cell growth by ∼30%.
Under 50% inhibitory or 30% stimulatory Na2SeO3 concentration, selenopolypeptide genes (fdnG, fdoG, and fdhF) whose products require SeCys but not SeCys biosynthesis machinery genes were found to be induced ≥2-fold.
In addition, one sulfur (S) metabolic gene iscS and two previously reported selenite-responsive genes sodA and gutS were also induced ≥2-fold under 50% inhibitory concentration.
Our findings provide insight about the detoxification of selenite in E.
coli via induction of these genes involved in the selenite reduction process.
American Psychological Association (APA)
Tetteh, Antonia Y.& Sun, Katherine H.& Hung, Chiu-Yueh& Kittur, Farooqahmed S.& Ibeanu, Gordon C.& Williams, Daniel…[et al.]. 2014. Transcriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reduction. International Journal of Microbiology،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-468715
Modern Language Association (MLA)
Tetteh, Antonia Y.…[et al.]. Transcriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reduction. International Journal of Microbiology No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-468715
American Medical Association (AMA)
Tetteh, Antonia Y.& Sun, Katherine H.& Hung, Chiu-Yueh& Kittur, Farooqahmed S.& Ibeanu, Gordon C.& Williams, Daniel…[et al.]. Transcriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reduction. International Journal of Microbiology. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-468715
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468715