Repeated Intraperitoneal α-Radioimmunotherapy of Ovarian Cancer in Mice
Joint Authors
Lindegren, Sture
Jensen, Holger
Warnhammar, Elisabet
Kahu, Helena
Hultborn, Ragnar
Elgqvist, Jörgen
Andersson, Håkan
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-10-25
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The aim of this study was to investigate the therapeutic efficacy of α-radioimmunotherapy of ovarian cancer in mice using different fractionated treatment regimens.
The study was performed using the monoclonal antibody MX35 F(ab′)2 labeled with the α-particle emitter A211t.
Methods.
Nude mice were intraperitoneally inoculated with ~1×107 cells of the cell line NIH:OVCAR-3.
Four weeks later 6 groups of animals were given 400 kBq 211At-MX35 F(ab′)2 as a single or as a repeated treatment of up to 6 times (n=18 in each group).
The fractionated treatments were given every seventh day.
Control animals were treated with unlabeled MX35 F(ab′)2 (n=12).
Eight weeks posttreatment the animals were sacrificed and the presence of macro- and microscopic tumors and ascites was determined.
Results.
The tumor-free fractions (TFFs) of the animals, defined as the fraction of animals with no macro- and microtumors and no ascites, were 0.17, 0.11, 0.39, 0.44, 0.44, and 0.67 when treated with 400 kBq 211At-MX35 F(ab′)2 once or 2, 3, 4, 5, or 6 times, respectively.
Repeated treatment 3 times or more resulted in a significantly higher (P<.05) TFF than compared to treatment once or twice.
The presence of ascites decreased from 15 out of 18 animals in the group given only one treatment to zero for the 2 groups given 5 or 6 fractions.
Treatment with unlabeled MX35 F(ab′)2 resulted in a TFF of zero.
Conclusion.
Weekly repeated intraperitoneal injections of tolerable amounts of activity of A211t-MX35 F(ab′)2 of up to 6 times produced increased therapeutic efficacy without observed toxicity, indicating a potential increase of the therapeutic index.
American Psychological Association (APA)
Elgqvist, Jörgen& Andersson, Håkan& Jensen, Holger& Kahu, Helena& Lindegren, Sture& Warnhammar, Elisabet…[et al.]. 2009. Repeated Intraperitoneal α-Radioimmunotherapy of Ovarian Cancer in Mice. Journal of Oncology،Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-468724
Modern Language Association (MLA)
Elgqvist, Jörgen…[et al.]. Repeated Intraperitoneal α-Radioimmunotherapy of Ovarian Cancer in Mice. Journal of Oncology No. 2010 (2010), pp.1-5.
https://search.emarefa.net/detail/BIM-468724
American Medical Association (AMA)
Elgqvist, Jörgen& Andersson, Håkan& Jensen, Holger& Kahu, Helena& Lindegren, Sture& Warnhammar, Elisabet…[et al.]. Repeated Intraperitoneal α-Radioimmunotherapy of Ovarian Cancer in Mice. Journal of Oncology. 2009. Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-468724
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468724