Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1)‎ in Non-Candida albicans Candida (NCAC)‎ Species

Joint Authors

Jamil, Nur Alyaa
Wan Harun, Wan Himratul Aznita
Jamaludin, Nor Hazwani
Nordin, Mohd-Al-Faisal

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-06-18

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Medicine

Abstract EN

The study aimed to identify the HWP1 gene in non-Candida albicans Candida species and the differential expression of HWP1 following treatment with Piper betle and Brucea javanica aqueous extracts.

All candidal suspensions were standardized to 1×106 cells/mL.

The suspension was incubated overnight at 37 °C (C.

parapsilosis, 35°C).

Candidal cells were treated with each respective extract at 1, 3, and 6 mg/mL for 24 h.

The total RNA was extracted and reverse transcription-polymerase chain reaction was carried out with a specific primer of HWP1.

HWP1 mRNAs were only detected in C.

albicans, C.

parapsilosis, and C.

tropicalis.

Exposing the cells to the aqueous extracts has affected the expression of HWP1 transcripts.

C.

albicans, C.

parapsilosis, and C.

tropicalis have demonstrated different intensity of mRNA.

Compared to P.

betle, B.

javanica demonstrated a higher suppression on the transcript levels of HWP1 in all samples.

HWP1 was not detected in C.

albicans following the treatment of B.

javanica at 1 mg/mL.

In contrast, C.

parapsilosis and C.

tropicalis were shown to have HWP1 regulation.

However, the expression levels were reduced upon the addition of higher concentration of B.

javanica extract.

P.

betle and B.

javanica have potential to be developed as oral health product.

American Psychological Association (APA)

Wan Harun, Wan Himratul Aznita& Jamil, Nur Alyaa& Jamaludin, Nor Hazwani& Nordin, Mohd-Al-Faisal. 2013. Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-468921

Modern Language Association (MLA)

Wan Harun, Wan Himratul Aznita…[et al.]. Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-468921

American Medical Association (AMA)

Wan Harun, Wan Himratul Aznita& Jamil, Nur Alyaa& Jamaludin, Nor Hazwani& Nordin, Mohd-Al-Faisal. Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-468921

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-468921