Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species
Joint Authors
Jamil, Nur Alyaa
Wan Harun, Wan Himratul Aznita
Jamaludin, Nor Hazwani
Nordin, Mohd-Al-Faisal
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-18
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The study aimed to identify the HWP1 gene in non-Candida albicans Candida species and the differential expression of HWP1 following treatment with Piper betle and Brucea javanica aqueous extracts.
All candidal suspensions were standardized to 1×106 cells/mL.
The suspension was incubated overnight at 37 °C (C.
parapsilosis, 35°C).
Candidal cells were treated with each respective extract at 1, 3, and 6 mg/mL for 24 h.
The total RNA was extracted and reverse transcription-polymerase chain reaction was carried out with a specific primer of HWP1.
HWP1 mRNAs were only detected in C.
albicans, C.
parapsilosis, and C.
tropicalis.
Exposing the cells to the aqueous extracts has affected the expression of HWP1 transcripts.
C.
albicans, C.
parapsilosis, and C.
tropicalis have demonstrated different intensity of mRNA.
Compared to P.
betle, B.
javanica demonstrated a higher suppression on the transcript levels of HWP1 in all samples.
HWP1 was not detected in C.
albicans following the treatment of B.
javanica at 1 mg/mL.
In contrast, C.
parapsilosis and C.
tropicalis were shown to have HWP1 regulation.
However, the expression levels were reduced upon the addition of higher concentration of B.
javanica extract.
P.
betle and B.
javanica have potential to be developed as oral health product.
American Psychological Association (APA)
Wan Harun, Wan Himratul Aznita& Jamil, Nur Alyaa& Jamaludin, Nor Hazwani& Nordin, Mohd-Al-Faisal. 2013. Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-468921
Modern Language Association (MLA)
Wan Harun, Wan Himratul Aznita…[et al.]. Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-468921
American Medical Association (AMA)
Wan Harun, Wan Himratul Aznita& Jamil, Nur Alyaa& Jamaludin, Nor Hazwani& Nordin, Mohd-Al-Faisal. Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-468921
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468921