Proteomic Analysis of Serum in Lung Cancer Induced by 3-Methylcholanthrene
Joint Authors
Li, Minhua
Xia, Dong
Yang, Fei
Zhang, Yuxia
Ye, Bo
Liu, Mingqiu
Chen, Honglei
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-09-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Lung cancer remains the leading cause of cancer-related mortality worldwide.
Early detection of lung cancer is problematic due to the lack of a marker with high diagnosis sensitivity and specificity.
To determine the differently expressed proteins in the serum of lung cancer and figure out the function of the proteins, two-dimensional electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were used to screen the serum proteins of lung cancer model induced by 3-methylcholanthrene (MCA).
From optimized 2DE image, 455 spots in the normal sera and 716 spots in the lung cancers sera were detected.
Among them, 141 protein spots were differentially expressed when comparing the serum from normal rat and serum from lung cancer model, including 82 overexpressed proteins and 59 underexpressed proteins.
Changes of haptoglobin, transthyretin, and TNF superfamily member 8 (TNFRS8) were confirmed in sera from lung cancer by MALDI-TOF-MS.
Proteomics technology leads to identify changes of haptoglobin, transthyretin, and TNFRS8 in serum of rat lung cancer model and represents a powerful tool in searching for candidate proteins as biomarkers.
American Psychological Association (APA)
Li, Minhua& Ye, Bo& Zhang, Yuxia& Chen, Honglei& Xia, Dong& Liu, Mingqiu…[et al.]. 2009. Proteomic Analysis of Serum in Lung Cancer Induced by 3-Methylcholanthrene. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-468967
Modern Language Association (MLA)
Li, Minhua…[et al.]. Proteomic Analysis of Serum in Lung Cancer Induced by 3-Methylcholanthrene. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-12.
https://search.emarefa.net/detail/BIM-468967
American Medical Association (AMA)
Li, Minhua& Ye, Bo& Zhang, Yuxia& Chen, Honglei& Xia, Dong& Liu, Mingqiu…[et al.]. Proteomic Analysis of Serum in Lung Cancer Induced by 3-Methylcholanthrene. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-468967
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468967