Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons In Vitro
Joint Authors
Gómez-Serranillos, María Pilar
Arce, Carmen
El-Naggar, Tarek
Palomino, Olga María
Carretero, María Emilia
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Nigella sativa L.
(NS) has been used for medicinal purposes since ancient times.
This study aimed to investigate the cytotoxicity of NS dry methanolic extract on cultured cortical neurons and its influence on neurotransmitter release, as well as the presence of excitatory (glutamate and aspartate) and inhibitory amino acids (gamma-aminobutyric acid—GABA—and glycine) in NS extract.
Cultured rat cortical neurons were exposed to different times and concentrations of NS dry methanolic extract and cell viability was then determined by a quantitative colorimetric method.
NS did not induce any toxicity.
The secretion of different amino acids was studied in primary cultured cortical neurons by high-performance liquid chromatography (HPLC) using a derivation before injection with dansyl chloride.
NS modulated amino acid release in cultured neurons; GABA was significantly increased whereas secretion of glutamate, aspartate, and glycine were decreased.
The in vitro findings support the hypothesis that the sedative and depressive effects of NS observed in vivo could be based on changes of inhibitory/excitatory amino acids levels.
American Psychological Association (APA)
El-Naggar, Tarek& Gómez-Serranillos, María Pilar& Palomino, Olga María& Arce, Carmen& Carretero, María Emilia. 2010. Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons In Vitro. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-469005
Modern Language Association (MLA)
El-Naggar, Tarek…[et al.]. Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons In Vitro. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-469005
American Medical Association (AMA)
El-Naggar, Tarek& Gómez-Serranillos, María Pilar& Palomino, Olga María& Arce, Carmen& Carretero, María Emilia. Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons In Vitro. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-469005
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469005