Cell-Type-Specific Effects of Silibinin on Vitamin D-Induced Differentiation of Acute Myeloid Leukemia Cells Are Associated with Differential Modulation of RXRα Levels
Joint Authors
Danilenko, Michael
Wassermann, Rina
Novik, Victoria
Source
Leukemia Research and Treatment
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Mechanical Engineering
Medicine
Abstract EN
Plant polyphenols have been shown to enhance the differentiation of acute myeloid leukemia (AML) cells induced by the hormonal form of vitamin D3 (1α,25-dihydroxyvitamin D3; 1,25D).
However, how these agents modulate 1,25D effects in different subtypes of AML cells remains poorly understood.
Here, we show that both carnosic acid (CA) and silibinin (SIL) synergistically enhancd 1,25D-induced differentiation of myeloblastic HL60 cells.
However, in promonocytic U937 cells, only CA caused potentiation while SIL attenuated 1,25D effect.
The enhanced effect of 1,25D+CA was accompanied by increases in both the vitamin D receptor (VDR) and retinoid X receptor alpha (RXRα) protein levels and vitamin D response element (VDRE) transactivation in both cell lines.
Similar increases were observed in HL60 cells treated with 1,25D + SIL.
In U937 cells, however, SIL inhibited 1,25D-induced VDRE transactivation concomitant with downregulation of RXRα at both transcriptional and posttranscriptional levels.
These inhibitory effects correlated with the inability of SIL, with or without 1,25D, to activate the Nrf2/antioxidant response element signaling pathway in U937 cells.
These results suggest that opposite effects of SIL on 1,25D-induced differentiation of HL60 and U937 cells may be determined by cell-type-specific signaling and transcriptional responses to this polyphenol resulting in differential modulation of RXRα expression.
American Psychological Association (APA)
Wassermann, Rina& Novik, Victoria& Danilenko, Michael. 2012. Cell-Type-Specific Effects of Silibinin on Vitamin D-Induced Differentiation of Acute Myeloid Leukemia Cells Are Associated with Differential Modulation of RXRα Levels. Leukemia Research and Treatment،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-469139
Modern Language Association (MLA)
Wassermann, Rina…[et al.]. Cell-Type-Specific Effects of Silibinin on Vitamin D-Induced Differentiation of Acute Myeloid Leukemia Cells Are Associated with Differential Modulation of RXRα Levels. Leukemia Research and Treatment No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-469139
American Medical Association (AMA)
Wassermann, Rina& Novik, Victoria& Danilenko, Michael. Cell-Type-Specific Effects of Silibinin on Vitamin D-Induced Differentiation of Acute Myeloid Leukemia Cells Are Associated with Differential Modulation of RXRα Levels. Leukemia Research and Treatment. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-469139
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469139