Separation of cis- and trans-Asarone from Acorus tatarinowii by Preparative Gas Chromatography
Joint Authors
Zhang, X. M.
Zuo, H. L.
Xia, Z. N.
Yang, F. Q.
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-12
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A preparative gas chromatography (pGC) method was developed for the separation of isomers (cis- and trans-asarone) from essential oil of Acorus tatarinowii.
The oil was primarily fractionated by silica gel chromatography using different ratios of petroleum ether and ethyl acetate as gradient elution solvents.
And then the fraction that contains mixture of the isomers was further separated by pGC.
The compounds were separated on a stainless steel column packed with 10% OV-101 (3 m × 6 mm, i.d.), and then the effluent was split into two gas flows.
One percent of the effluent passed to the flame ionization detector (FID) for detection and the remaining 99% was directed to the fraction collector.
Two isomers were collected after 90 single injections (5 uL) with the yield of 178 mg and 82 mg, respectively.
Furthermore, the structures of the obtained compounds were identified as cis- and trans-asarone by 1H- and 13C-NMR spectra, respectively.
American Psychological Association (APA)
Zuo, H. L.& Yang, F. Q.& Zhang, X. M.& Xia, Z. N.. 2012. Separation of cis- and trans-Asarone from Acorus tatarinowii by Preparative Gas Chromatography. Journal of Analytical Methods in Chemistry،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-469166
Modern Language Association (MLA)
Zuo, H. L.…[et al.]. Separation of cis- and trans-Asarone from Acorus tatarinowii by Preparative Gas Chromatography. Journal of Analytical Methods in Chemistry No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-469166
American Medical Association (AMA)
Zuo, H. L.& Yang, F. Q.& Zhang, X. M.& Xia, Z. N.. Separation of cis- and trans-Asarone from Acorus tatarinowii by Preparative Gas Chromatography. Journal of Analytical Methods in Chemistry. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-469166
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469166