Gleditsia sinensis : Transcriptome Sequencing, Construction, and Application of Its Protein-Protein Interaction Network
Joint Authors
Guo, Wenna
Zhu, Liucun
Zhang, Ying
Wang, Qiang
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Gleditsia sinensis is a genus of deciduous tree in the family Caesalpinioideae, native to China, and is of great economic importance.
However, despite its economic value, gene sequence information is strongly lacking.
In the present study, transcriptome sequencing of G.
sinensis was performed resulting in approximately 75.5 million clean reads assembled into 142155 unique transcripts generating 58583 unigenes.
The average length of the unigenes was 900 bp, with an N50 of 549 bp.
The obtained unigene sequences were then compared to four protein databases to include NCBI nonredundant protein (NRDB), Swiss-prot, Kyoto Encyclopedia of Genes and Genomes (KEGG), and the Cluster of Orthologous Groups (COG).
Using BLAST procedure, 31385 unigenes (53.6%) were generated to have functional annotations.
Additionally, sequence homologies between identified unigenes and genes of known species in a protein-protein interaction (PPI) network facilitated G.
sinensis PPI network construction.
Based on this network construction, new stress resistance genes (including cold, drought, and high salinity) were predicted.
The present study is the first investigation of genome-wide gene expression in G.
sinensis with the results providing a basis for future functional genomic studies relating to this species.
American Psychological Association (APA)
Zhu, Liucun& Zhang, Ying& Guo, Wenna& Wang, Qiang. 2014. Gleditsia sinensis : Transcriptome Sequencing, Construction, and Application of Its Protein-Protein Interaction Network. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-469386
Modern Language Association (MLA)
Zhu, Liucun…[et al.]. Gleditsia sinensis : Transcriptome Sequencing, Construction, and Application of Its Protein-Protein Interaction Network. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-469386
American Medical Association (AMA)
Zhu, Liucun& Zhang, Ying& Guo, Wenna& Wang, Qiang. Gleditsia sinensis : Transcriptome Sequencing, Construction, and Application of Its Protein-Protein Interaction Network. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-469386
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469386