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Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Codeine Phosphate and Chlorpheniramine Maleate from Their Combined Liquid Dosage Form
Joint Authors
Basappa, Praveen
Kommana, Ramakrishna
Source
Chromatography Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The present paper describes the development of quick stability indicating RP-HPLC method for the simultaneous estimation of codeine phosphate and chlorpheniramine maleate in the presence of its degradation products, generated from forced degradation studies.
The developed method separates codeine phosphate and chlorpheniramine maleate in impurities/degradation products.
Codeine phosphate and chlorpheniramine maleate and their combination drug product were exposed to acid, base, oxidation, dry heat, and photolytic stress conditions, and the stressed samples were analysed by proposed method.
The proposed HPLC method utilizes the Shimadzu HPLC system on a Phenomenex C18 column (250 mm×4.6 mm, 5 μ) using a mixture of 1% o-phosphoric acid in water : acetonitrile : methanol (78 : 10 : 12) mobile phase with pH adjusted to 3.0 in an isocratic elution mode at a flow rate of 1 mL/min, at 23°C with a load of 20 μL.
The detection was carried out at 254 nm.
The retention time of codeine phosphate and chlorpheniramine maleate was found to be around 3.47 min and 9.45 min, respectively.
The method has been validated with respect to linearity, robustness, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ).
The developed validated stability indicating HPLC method was found to be simple, accurate, and reproducible for the determination of instability of these drugs in bulk and commercial products.
American Psychological Association (APA)
Kommana, Ramakrishna& Basappa, Praveen. 2013. Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Codeine Phosphate and Chlorpheniramine Maleate from Their Combined Liquid Dosage Form. Chromatography Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-469406
Modern Language Association (MLA)
Kommana, Ramakrishna& Basappa, Praveen. Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Codeine Phosphate and Chlorpheniramine Maleate from Their Combined Liquid Dosage Form. Chromatography Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-469406
American Medical Association (AMA)
Kommana, Ramakrishna& Basappa, Praveen. Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Codeine Phosphate and Chlorpheniramine Maleate from Their Combined Liquid Dosage Form. Chromatography Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-469406
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469406