Long Non-Coding RNA Deregulation in Tongue Squamous Cell Carcinoma
Joint Authors
Wong, Thian-Sze
Gao, Wei
Chan, Jimmy Yu-Wai
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
The deregulated tumorigenic long non-coding RNA (lncRNA) has been reported in several malignancies.
However, there is still no comprehensive study on tongue squamous cell carcinoma (SCC).
Methods.
Functional reannotation for the human lncRNA was carried out by ncFANs.
Real-time quantitative PCR was used to validate the identified lncRNAs.
Results.
Using the functional annotation algorithm from ncFANs, 8 differentially expressed lncRNAs were identified.
Lnc-PPP2R4-5, lnc-SPRR2D-1, lnc-MAN1A2-1, lnc-FAM46A-1, lnc-MBL2-4:1, and lnc-MBL2-4:3 were upregulated in the microdissected tongue SCC tissues.
In comparison, lnc-AL355149.1-1 and lnc-STXBP5-1 showed significant downregulation.
High level of lnc-MBL2-4:3 was significantly associated with the node positive tongue SCC patients.
Further, patients with advanced T-stage demonstrated a further reduction of lnc-AL355149.1-1 in the tumor tissues.
Treatment of tongue SCC cells with 5-fluorouracil and paclitaxel can reserve the expression patterns observed in the tongue SCC tissues.
Further, changes of lnc-MBL2-4:3 and lnc-AL355149.1-1 expression levels were noticed in the cisplatin-resistant tongue SCC cells.
Conclusions.
Our results demonstrated that functional reannotation allows us to identify novel lncRNAs using the existing gene expression array dataset.
The association of lncRNA with the T-stage and nodal status of tongue SCC patients suggested that lncRNA deregulation was involved in the pathogenesis of tongue SCC.
American Psychological Association (APA)
Gao, Wei& Chan, Jimmy Yu-Wai& Wong, Thian-Sze. 2014. Long Non-Coding RNA Deregulation in Tongue Squamous Cell Carcinoma. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-469518
Modern Language Association (MLA)
Gao, Wei…[et al.]. Long Non-Coding RNA Deregulation in Tongue Squamous Cell Carcinoma. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-469518
American Medical Association (AMA)
Gao, Wei& Chan, Jimmy Yu-Wai& Wong, Thian-Sze. Long Non-Coding RNA Deregulation in Tongue Squamous Cell Carcinoma. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-469518
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469518