Enhancement of Human Prolactin Synthesis by Sodium Butyrate Addition to Serum-Free CHO Cell Culture
Joint Authors
Soares, Carlos Roberto Jorge
Rodrigues Goulart, Herbert
Arthuso, Fernanda dos Santos
Capone, Marcos Vinicius Nucci
Bartolini, Paolo
Oliveira, Taís Lima de
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Sodium butyrate (NaBu) has been used as a productivity enhancer for the synthesis of recombinant proteins in Chinese hamster ovary (CHO) cells.
Thus, the influence of NaBu on the production of recombinant human prolactin (hPRL) from CHO cells was investigated for the first time.
CHO cell cultures were submitted to a treatment with different concentrations of NaBu (0.25 to 4 mM).
Quantitative and qualitative analyses by reverse-phase high-performance liquid chromatography (RP-HPLC) and Western blot or SDS-PAGE, carried out directly on CHO-conditioned medium, showed that the highest hPRL expression was obtained with 1 mM NaBu.
In vitro biological assays based on noble rat lymphoma (Nb2) and mouse pro-B lymphoma (Ba/F3-LLP) cells were carried out on purified hPRL.
Its bioactivity in the presence of NaBu was not apparently different from that of the First International Reference Reagent of recombinant hPRL (WHO 97/714).
Our results show that NaBu increased the synthesis of recombinant hPRL in CHO cells, apparently without compromising either its structure or function.
American Psychological Association (APA)
Rodrigues Goulart, Herbert& Arthuso, Fernanda dos Santos& Capone, Marcos Vinicius Nucci& Oliveira, Taís Lima de& Bartolini, Paolo& Soares, Carlos Roberto Jorge. 2010. Enhancement of Human Prolactin Synthesis by Sodium Butyrate Addition to Serum-Free CHO Cell Culture. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-469519
Modern Language Association (MLA)
Rodrigues Goulart, Herbert…[et al.]. Enhancement of Human Prolactin Synthesis by Sodium Butyrate Addition to Serum-Free CHO Cell Culture. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-469519
American Medical Association (AMA)
Rodrigues Goulart, Herbert& Arthuso, Fernanda dos Santos& Capone, Marcos Vinicius Nucci& Oliveira, Taís Lima de& Bartolini, Paolo& Soares, Carlos Roberto Jorge. Enhancement of Human Prolactin Synthesis by Sodium Butyrate Addition to Serum-Free CHO Cell Culture. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-469519
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469519