Serendipitous Isolation of Non-Vibrio Bacterial Strains Carrying the Cholera Toxin Gene from Environmental Waters in Indonesia
Joint Authors
Nomoto, Ryohei
de Vries, Garry Cores
Osawa, Ro
Shibata, Yusuke
Source
International Journal of Microbiology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
We initially attempted to isolate a Vibrio cholerae O1 El Tor biotype that carries a novel variant of the cholera toxin gene (ctxAB) from environmental waters of Indonesia, where the seventh cholera pandemic by V.
cholerae O1 El Tor biotype began.
Nested PCR targeting the gene revealed that a total of eight strains were found to carry ctxAB.
However, sequencing of the 16S rRNA genes of these isolates showed they were not V.
cholerae but were either Klebsiella, Enterobacter, Pantoea, or Aeromonas.
Subsequent nested PCR assays targeting all genes known to be encoded on the CTX phage (i.e., zot, ace, orfU, cep, rstB, rstA, and rstR) showed that one isolate belonged to the Enterobacter genus carried all the genes tested, while the other isolates lacked either 2, 3, or 5 of the genes.
This evidence suggests that phages with ctxAB are genetically diverse and can infect not only V.
cholerae and V.
mimicus but also other species and genera in the form of a pseudolysogen.
American Psychological Association (APA)
Shibata, Yusuke& Nomoto, Ryohei& de Vries, Garry Cores& Osawa, Ro. 2013. Serendipitous Isolation of Non-Vibrio Bacterial Strains Carrying the Cholera Toxin Gene from Environmental Waters in Indonesia. International Journal of Microbiology،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-469539
Modern Language Association (MLA)
Shibata, Yusuke…[et al.]. Serendipitous Isolation of Non-Vibrio Bacterial Strains Carrying the Cholera Toxin Gene from Environmental Waters in Indonesia. International Journal of Microbiology No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-469539
American Medical Association (AMA)
Shibata, Yusuke& Nomoto, Ryohei& de Vries, Garry Cores& Osawa, Ro. Serendipitous Isolation of Non-Vibrio Bacterial Strains Carrying the Cholera Toxin Gene from Environmental Waters in Indonesia. International Journal of Microbiology. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-469539
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469539