CCAATEnhancer-Binding Protein α Is a Crucial Regulator of Human Fat Mass and Obesity Associated Gene Transcription and Expression
Joint Authors
Ding, Ying
Ren, Wei
Lu, Jun
Jia, Weiping
Liang, Xiubin
Jiang, Feng
Li, Aimei
Guo, Jianjin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Several susceptibility loci have been reported associated with obesity and T2DM in GWAS.
Fat mass and obesity associated gene (FTO) is the first gene associated with body mass index (BMI) and risk for diabetes in diverse patient populations.
FTO is highly expressed in the brain and pancreas, and is involved in regulating dietary intake and energy expenditure.
While much is known about the epigenetic mutations contributing to obesity and T2DM, less is certain with the expression regulation of FTO gene.
In this study, a highly conserved canonical C/EBPα binding site was located around position −45~−54 bp relative to the human FTO gene transcriptional start site.
Site-directed mutagenesis of the putative C/EBPα binding sites decreased FTO promoter activity.
Overexpression and RNAi studies also indicated that C/EBPα was required for the expression of FTO.
Chromatin immunoprecipitation (ChIP) experiment was carried out and the result shows direct binding of C/EBPα to the putative binding regions in the FTO promoter.
Collectively, our data suggest that C/EBPα may act as a positive regulator binding to FTO promoter and consequently, activates the gene transcription.
American Psychological Association (APA)
Ren, Wei& Guo, Jianjin& Jiang, Feng& Lu, Jun& Ding, Ying& Li, Aimei…[et al.]. 2014. CCAATEnhancer-Binding Protein α Is a Crucial Regulator of Human Fat Mass and Obesity Associated Gene Transcription and Expression. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-469632
Modern Language Association (MLA)
Ren, Wei…[et al.]. CCAATEnhancer-Binding Protein α Is a Crucial Regulator of Human Fat Mass and Obesity Associated Gene Transcription and Expression. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-469632
American Medical Association (AMA)
Ren, Wei& Guo, Jianjin& Jiang, Feng& Lu, Jun& Ding, Ying& Li, Aimei…[et al.]. CCAATEnhancer-Binding Protein α Is a Crucial Regulator of Human Fat Mass and Obesity Associated Gene Transcription and Expression. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-469632
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469632