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Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA
Joint Authors
Motorin, Yuri
Helm, Mark
Behm-Ansmant, Isabelle
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-13
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Naturally occurring cellular RNAs contain an impressive number of chemically distinct modified residues which appear posttranscriptionally, as a result of specific action of the corresponding RNA modification enzymes.
Over 100 different chemical modifications have been identified and characterized up to now.
Identification of the chemical nature and exact position of these modifications is typically based on 2D-TLC analysis of nucleotide digests, on HPLC coupled with mass spectrometry, or on the use of primer extension by reverse transcriptase.
However, many modified nucleotides are silent in reverse transcription, since the presence of additional chemical groups frequently does not change base-pairing properties.
In this paper, we give a summary of various chemical approaches exploiting the specific reactivity of modified nucleotides in RNA for their detection.
American Psychological Association (APA)
Behm-Ansmant, Isabelle& Helm, Mark& Motorin, Yuri. 2011. Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA. Journal of Nucleic Acids،Vol. 2011, no. 2011, pp.1-17.
https://search.emarefa.net/detail/BIM-469751
Modern Language Association (MLA)
Behm-Ansmant, Isabelle…[et al.]. Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA. Journal of Nucleic Acids No. 2011 (2011), pp.1-17.
https://search.emarefa.net/detail/BIM-469751
American Medical Association (AMA)
Behm-Ansmant, Isabelle& Helm, Mark& Motorin, Yuri. Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA. Journal of Nucleic Acids. 2011. Vol. 2011, no. 2011, pp.1-17.
https://search.emarefa.net/detail/BIM-469751
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469751