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Interaction of Avelox with Bovine Serum Albumin and Effect of the Coexistent Drugs on the Reaction
Joint Authors
Yang, Chao
Yan, Xiaona
Lü, Yunkai
Wang, Jing
Liu, Baosheng
Source
International Journal of Analytical Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The interaction between Avelox and bovine serum albumin (BSA) was investigated at different temperatures by fluorescence spectroscopy.
Results showed that Avelox could quench the intrinsic fluorescence of BSA strongly, and the quenching mechanism was a static quenching process with Förester spectroscopy energy transfer.
The electrostatic force played an important role on the conjugation reaction between BSA and Avelox.
The order of magnitude of binding constants (Ka) was 104, and the number of binding site (n) in the binary system was approximately equal to 1.
The binding distance (r) was less than 3 nm and the primary binding site for Avelox was located in subdomain IIA of BSA.
Synchronous fluorescence spectra clearly revealed that the microenvironment of amino acid residues and the conformation of BSA were changed during the binding reaction.
In addition, the effect of some antibiotics on the binding constant of Avelox with BSA was also studied.
American Psychological Association (APA)
Liu, Baosheng& Yang, Chao& Yan, Xiaona& Wang, Jing& Lü, Yunkai. 2012. Interaction of Avelox with Bovine Serum Albumin and Effect of the Coexistent Drugs on the Reaction. International Journal of Analytical Chemistry،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-469752
Modern Language Association (MLA)
Liu, Baosheng…[et al.]. Interaction of Avelox with Bovine Serum Albumin and Effect of the Coexistent Drugs on the Reaction. International Journal of Analytical Chemistry No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-469752
American Medical Association (AMA)
Liu, Baosheng& Yang, Chao& Yan, Xiaona& Wang, Jing& Lü, Yunkai. Interaction of Avelox with Bovine Serum Albumin and Effect of the Coexistent Drugs on the Reaction. International Journal of Analytical Chemistry. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-469752
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469752