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Proteomic Profiling of the Dioxin-Degrading Bacterium Sphingomonas wittichii RW1
Joint Authors
Colquhoun, David R.
Halden, Rolf U.
Hartmann, Erica M.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Sphingomonas wittichii RW1 is a bacterium of interest due to its ability to degrade polychlorinated dioxins, which represent priority pollutants in the USA and worldwide.
Although its genome has been fully sequenced, many questions exist regarding changes in protein expression of S.
wittichii RW1 in response to dioxin metabolism.
We used difference gel electrophoresis (DIGE) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to identify proteomic changes induced by growth on dibenzofuran, a surrogate for dioxin, as compared to acetate.
Approximately 10% of the entire putative proteome of RW1 could be observed.
Several components of the dioxin and dibenzofuran degradation pathway were shown to be upregulated, thereby highlighting the utility of using proteomic analyses for studying bioremediation agents.
This is the first global protein analysis of a microorganism capable of utilizing the carbon backbone of both polychlorinated dioxins and dibenzofurans as the sole source for carbon and energy.
American Psychological Association (APA)
Colquhoun, David R.& Hartmann, Erica M.& Halden, Rolf U.. 2012. Proteomic Profiling of the Dioxin-Degrading Bacterium Sphingomonas wittichii RW1. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-469821
Modern Language Association (MLA)
Colquhoun, David R.…[et al.]. Proteomic Profiling of the Dioxin-Degrading Bacterium Sphingomonas wittichii RW1. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-469821
American Medical Association (AMA)
Colquhoun, David R.& Hartmann, Erica M.& Halden, Rolf U.. Proteomic Profiling of the Dioxin-Degrading Bacterium Sphingomonas wittichii RW1. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-469821
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469821