Whole Ovine Ovaries as a Model for Human : Perfusion with Cryoprotectants In Vivo and In Vitro
Joint Authors
Baikoshkarova, Saltanat
Dattena, Maria
Shalakhmetova, Tamara
Rahimi, Gohar
Mallmann, Peter
Isachenko, Evgenia
Kellerwessel, Elisabeth
Isachenko, Vladimir
Otarbaev, Marat
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
These experiments were performed to test the perfusion of ovine as a model for human ovaries by cryoprotectants in vivo at high temperature when the permeability of capillaries is high and when blood is insensibly replaced by the solution of cryoprotectants.
By our hypothetical supposition, ovaries could be saturated by cryoprotectants before their surgical removal.
The objective was to examine the effectiveness of perfusion of ovine ovaries with vascular pedicle in vivo and in vitro.
Arteria ovarica was cannuled and ovaries were perfused by Leibovitz L-15 medium + 100 IU/mL heparin + 5% bovine calf serum + 6% dimethyl sulfoxide + 6% ethylene glycol + 0.15 M sucrose + Indian ink in vivo and in vitro.
In the first and second cycle of experiments, ovaries (n=13 and n=23) were perfused in vivo and in vitro, respectively, during 60 min with the rate of perfusion 50 mL/h (0.8 mL/min).
It was established with in vivo perfusion that only about 10% of ovarian tissues were perfused due to an appearance of multiple anastomoses when the perfusion medium goes from arteria ovarica to arteria uterina without inflow into the ovaries.
It was concluded that in vitro perfusion of ovine intact ovaries with vascular pedicle by freezing medium is more effective than this manipulation performed in vivo.
American Psychological Association (APA)
Isachenko, Vladimir& Rahimi, Gohar& Dattena, Maria& Mallmann, Peter& Baikoshkarova, Saltanat& Kellerwessel, Elisabeth…[et al.]. 2014. Whole Ovine Ovaries as a Model for Human : Perfusion with Cryoprotectants In Vivo and In Vitro. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-469839
Modern Language Association (MLA)
Isachenko, Vladimir…[et al.]. Whole Ovine Ovaries as a Model for Human : Perfusion with Cryoprotectants In Vivo and In Vitro. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-469839
American Medical Association (AMA)
Isachenko, Vladimir& Rahimi, Gohar& Dattena, Maria& Mallmann, Peter& Baikoshkarova, Saltanat& Kellerwessel, Elisabeth…[et al.]. Whole Ovine Ovaries as a Model for Human : Perfusion with Cryoprotectants In Vivo and In Vitro. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-469839
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469839